Recombinant Human CD166 protein (Active) (ab288795)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
製品の詳細
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製品名
Recombinant Human CD166 protein (Active)
CD166 タンパク質・ペプチド 製品一覧 -
生理活性
Loaded Human CD6, Human Fc Tag on Protein A Biosensor can bind Human CD166/ALCAM, untagged with an affinity constant of 0.27 uM as determined in BLI assay (GatorBio Prime).
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精製度
>= 95 % SDS-PAGE. -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
Yes -
由来
Recombinant -
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生物種
Human -
配列
WYTVNSAYGDTIIIPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKS VQYDDVPEYKDRLNLSENYTLSISNARISDEKRFVCMLVTEDNVFEAPTI VKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVL HPLEGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGP SGQKTIHSEQAVFDIYYPTEQVTIQVLPPKNAIKEGDNITLKCLGNGNPP PEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKKSMIASTAI TVHYLDLSLNPSGEVTRQIGDALPVSCTISASRNATVVWMKDNIRLRSSP SFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKMTKKTDPS GLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISP EENVTLTCTAENQLERTVNSLNVSAISIPEHDEADEISDENREKVNDQAK -
予測される分子量
65 kDa -
領域
28 to 527 -
配列の追加情報
N-terminal glycine
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製品の詳細
Recombinant Human CD166 protein
特性
Our Abpromise guarantee covers the use of ab288795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
関連情報
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別名
- Activated leukocyte cell adhesion molecule
- ALCAM
- ALCAM protein
see all -
機能
Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. -
組織特異性
Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines. -
配列類似性
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
ドメイン
The CD6 binding site is located in the N-terminal Ig-like domain. -
細胞内局在
Membrane. - Information by UniProt
画像
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Biological Activity - Recombinant Human CD166 protein (Active) (ab288795)
Loaded Human CD6, Human Fc Tag on Protein A Biosensor can bind Human CD166/ALCAM, untagged with an affinity constant of 0.27 uM as determined in BLI assay (GatorBio Prime).
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SDS-PAGE - Recombinant Human CD166 protein (Active) (ab288795)SDS-PAGE analysis of ab288795 -
Mass Spectrometry - Recombinant Human CD166 protein (Active) (ab288795)Mass determination by ESI-TOF.
Predicted MW is 56128.61 Da. (+/- 10 Da by ESI-TOF). Observed MW is 56007.91 Da. M -121 Due to C-terminal Lysine cleavage.
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HPLC - Recombinant Human CD166 protein (Active) (ab288795)HPLC analysis of ab288795
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab288795 は論文での使用が確認できていません。
