Recombinant Human CD137 protein (Active) (ab287936)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, MS
製品の詳細
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製品名
Recombinant Human CD137 protein (Active)
CD137 タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by the dose dependent inhibition of anti-4-1BB antibody mediated activation of genetically engineered Jurkat T cell coexpressing 4-1BB and a responsive reporter element in presence of FcgammaRIIb CHO-K1 cells.
ED50 is ≤ 126.7 ng/ml, corresponding to a specific activity of 7.9 x 103 units/mg.
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精製度
>= 95 % SDS-PAGE. -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Human -
配列
LQDPCSNCPAGTFCDNNRNQICSPCPPNSFSSAGGQRTCDICRQCKGVFR TRKECSSTSNAECDCTPGFHCLGAGCSMCEQDCKQGQELTKKGCKDCCFG TFNDQKRGICRPWTNCSLDGKSVLVNGTKERDVVCGPSPADLSPGASSVT PPAPAREPGHSPQ -
予測される分子量
17 kDa -
実際の分子量
17 kDa -
分子量情報
Mass determination by ESI TOF: Predicted MW is 17336.42 +/- 10Da. Observed MW is 17337.75 Da. -
領域
24 to 186 -
配列の追加情報
N-terminal glycine.
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特性
Our Abpromise guarantee covers the use of ab287936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Mass Spectrometry
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.4
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with phosphate buffered saline. Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product.
関連情報
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別名
- 4 1BB
- 4 1BB ligand receptor
- 4-1BB ligand receptor
see all -
機能
Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation. -
組織特異性
Expressed on the surface of activated T-cells. -
配列類似性
Contains 4 TNFR-Cys repeats. -
細胞内局在
Membrane. - Information by UniProt
画像
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Recombinant Human CD137 protein (Active) (ab287936)
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SDS-PAGE - Recombinant Human CD137 protein (Active) (ab287936)SDS-PAGE analysis of ab287936.
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Mass Spectrometry - Recombinant Human CD137 protein (Active) (ab287936)Mass spectometry analysis of ab287936
Mass determination by ESI TOF: 17336 +/-10Da. Observed mass is 17337.75 Da.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab287936 は論文での使用が確認できていません。
