AB227418

Recombinant human CD116 protein (Active)

Bioactive
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Recombinant human CD116 protein (Active) is a Human Fragment protein, in the 20 to 320 aa range, expressed in Baculovirus infected insect cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

別名を表示する

CD116, CSF2R, CSF2RY, CSF2RA, Granulocyte-macrophage colony-stimulating factor receptor subunit alpha, GM-CSF-R-alpha, GMCSFR-alpha, GMR-alpha, CDw116

1 Images

SDS-PAGE - Recombinant human CD116 protein (Active) (AB227418)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human CD116 protein (Active) (AB227418)

15% SDS-PAGE analysis of 3 μg ab227418.

MW 40-57 kDa (SDS-PAGE under reducing conditions).

Key facts

精製度

>90% SDS-PAGE

エンドトキシンレベル

< 1 EU/µg

発現系

Baculovirus infected insect cells

アプリケーション

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

Measured by its ability to inhibit GM-CSF dependent proliferation of TF1 human erythroleukemic cells. The ED₅₀ for this effect is less or equal to 10 μg/ml in the presence of 0.5 ng/ml GM-CSF.

アクセッション番号

P15509

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

This product was previously labelled as GM-CSF Receptor alpha

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配列情報

[{"sequence":"ADPLIPEKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTDKKNRVVEPRLSNNECSCTFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGREGTAAQNFSCFIYNADLMNCTWARGPTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREIGIQFFDSLLDTKKIERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQYQLDVHRKNTQPGTENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAIEFGSDDGHHHHHH","proteinLength":"Fragment","predictedMolecularWeight":"35.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":320,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"Baculovirus infected insect cells","accessionNumber":"P15509","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD116 also known as the CSF2RA (colony-stimulating factor 2 receptor alpha subunit) is a protein that plays an important role in cytokine receptor signaling. It has a mass of approximately 43 kDa. This receptor is widely expressed on the surface of hematopoietic cells including monocytes granulocytes and some T-lymphocytes. Furthermore CD116 presents important interactions with cellular functioning by being part of a heterodimeric receptor complex. It couples with the beta subunit (CSF2RB) together mediating effects of granulocyte-macrophage colony-stimulating factor (GM-CSF).

Biological function summary

The CSF2RA is integral in facilitating cellular communication. It belongs to the cytokine receptor family specifically binding the GM-CSF. This molecule triggers signaling cascades that are essential for the proliferation differentiation and survival of various blood cells. When GM-CSF binds to the receptor complex made of CD116 and CSF2RB it activates downstream pathways influencing these critical processes thereby ensuring proper bone marrow function and immune cell activity.

Pathways

CD116 participates in significant signal transduction cascades. It is involved in the JAK-STAT pathway which plays an important role in regulating immune responses and hematopoiesis. The engagement of GM-CSF with CD116 triggers the activation of JAK kinases followed by STAT protein phosphorylation. Another pathway linked with CD116 is the MAPK pathway which further mediates downstream signals that modulate cellular growth and differentiation integrating input from various activating signals.

Abnormalities in CD116 expression and function associate with several health conditions. One example is chronic myelomonocytic leukemia (CMML) where deregulated signaling through the GM-CSF receptor impacts cell proliferation and differentiation. Aberrant CD116 activity also ties into inflammation and autoimmune disorders such as rheumatoid arthritis where overactive signaling can worsen inflammatory responses. Proteins like GM-CSF and its beta receptor subunit CSF2RB accompany these disorders by contributing to the pathway dysregulation observed in these disease states.

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製品の性状

製品の状態

Liquid

補足情報

Affinity purified

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一般的な情報

機能

Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.

配列の類似性

Belongs to the type I cytokine receptor family. Type 5 subfamily.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.

See full target information CSF2RA

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Abcam product promise

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Recombinant human CD116 protein (Active)