Recombinant Human CCR8 protein (VLPs) (Active) (ab300191)
Key features and details
- Expression system: Mammalian
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: WB, ELISA
製品の詳細
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製品名
Recombinant Human CCR8 protein (VLPs) (Active)
CCR8 タンパク質・ペプチド 製品一覧 -
生理活性
The CCR8 protein is biologically active as it can react with the anti-CCR8 recombinant antibody. Activity of ab300191 was measured by its binding ability in a functional ELISA. EC50 is 11.13-17.29 ng/ml
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エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Mammalian -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MDYTLDLSVTTVTDYYYPDIFSSPCDAELIQTNGKLLLAVFYCLLFVFSL LGNSLVILVLVVCKKLRSITDVYLLNLALSDLLFVFSFPFQTYYLLDQWV FGTVMCKVVSGFYYIGFYSSMFFITLMSVDRYLAVVHAVYALKVRTIRMG TTLCLAVWLTAIMATIPLLVFYQVASEDGVLQCYSFYNQQTLKWKIFTNF KMNILGLLIPFTIFMFCYIKILHQLKRCQNHNKTKAIRLVLIVVIASLLF WVPFNVVLFLTSLHSMHILDGCSISQQLTYATHVTEIISFTHCCVNPVIY AFVGEKFKKHLSEIFQKSCSQIFNYLGRQMPRESCEKSSSCQQHSSRSSS VDYIL -
予測される分子量
42 kDa -
領域
1 to 355 -
タグ
His tag C-Terminus -
配列の追加情報
C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
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製品の詳細
Recombinant Human CCR8 Active protein (VLPs)
特性
Our Abpromise guarantee covers the use of ab300191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
ELISA
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製品の状態
Liquid -
備考
Recombinant full-length human CCR8 protein was expressed by the intact DNA sequence of the human CCR8 in mammalian cells. This recombinant CCR8 protein was fused with a 10xHis-tag at the C-terminus. It contains less than 1.0 EU/ug determined by the LAL method. The CCR8 protein is biologically active as it can react with the mouse anti-6xHis monoclonal antibody and anti-CCR8 recombinant antibody, respectively. Its VLP-like structure was observed and confirmed by TEM. This recombinant CCR8 protein is highly immunogenic because it carries viral protein.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 6% Trehalose, 94% PBSThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- C C chemokine receptor type 8
- C C CKR 8
- C C motif chemokine receptor 8
see all -
機能
Receptor for the chemokine CCL1/SCYA1/I-309. May regulate monocyte chemotaxis and thymic cell line apoptosis. Alternative coreceptor with CD4 for HIV-1 infection. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
画像
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Western blot - Recombinant Human CCR8 protein (VLPs) (Active) (AB300191)
AB300191 is detected by Mouse anti-6*His monoclonal antibody.
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Western blot - Recombinant Human CCR8 protein (VLPs) (Active) (AB300191)Ab300191 is detected by Anti-CCR8 recombinant Antibody.
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ELISA - Recombinant Human CCR8 protein (VLPs) (Active) (AB300191)Ab300191 activity measured by its binding ability in a functional ELISA. Immobilized human CCR8 (ab300191) at 5 μg/ml can bind Anti-CCR8 recombinant antibody, the EC50 is 11.13-17.29 ng/ml.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab300191 は論文での使用が確認できていません。
