Recombinant Human CCL27 protein (Active) (ab287948)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: HPLC, MS, SDS-PAGE
製品の詳細
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製品名
Recombinant Human CCL27 protein (Active)
CCL27 タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by the dose dependent measurement of beta-arrestin recruitment on CCR10 using U2OS overexpressing human CCR10/beta-arrestin/beta-galactosidase complementation system.
ED50 is ≤ 295 ng/ml, corresponding to a specific activity of 3.4 x 103 units/mg.
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精製度
>= 95 % SDS-PAGE.
>= 95 % HPLC -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Human -
配列
FLLPPSTACCTQLYRKPLSDKLLRKVIQVELQEADGDCHLQAFVLHLAQR SICIHPQNPSLSQWFEHQERKLHGTLPKLNFGMLRKMG -
予測される分子量
10 kDa -
実際の分子量
10 kDa -
分子量情報
Mass determination by ESI TOF: Predicted MW is 10206.97+/-10 Da. 41.42 +/- 10Da . Observed MW is 10207.41 -
領域
25 to 112 -
配列の追加情報
N-terminal glycine. Full-length mature chain lacking the signal peptide and pro-peptide.
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特性
Our Abpromise guarantee covers the use of ab287948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
HPLC
Mass Spectrometry
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.4
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with phosphate buffered saline. Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product.
関連情報
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別名
- ALP
- C-C motif chemokine 27
- CC chemokine ILC
see all -
機能
Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10. -
組織特異性
Testis, thymus, placenta, ovary and skin. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
細胞内局在
Secreted. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human CCL27 protein (ab287948)
SDS-PAGE analysis of ab287948
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Mass Spectrometry - Recombinant Human CCL27 protein (ab287948)Mass determination by ESI TOF: 10206.97+/- 10Da. Observed MW is 10207.41 Da.
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HPLC - Recombinant Human CCL27 protein (ab287948)HPLC analysis of ab287848
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Biological Activity - Recombinant Human CCL27 protein (Active) (ab287948)Fully biologically active determined by the dose dependent measurement of beta-arrestin recruitment on CCR10 using U2OS overexpressing human CCR10/beta-arrestin/beta-galactosidase complementation system.
ED50 is ≤ 295 ng/ml, corresponding to a specific activity of 3.4 x 103 units/mg.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab287948 は論文での使用が確認できていません。
