JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB283922

Recombinant Human CCL13 Protein (Active)

Recombinant Human CCL13 Protein (Active)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human CCL13 Protein (Active) is a Human Full Length protein, in the 24 to 98 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, FuncS, HPLC.

別名を表示する

MCP4, NCC1, SCYA13, CCL13, C-C motif chemokine 13, CK-beta-10, Monocyte chemoattractant protein 4, Monocyte chemotactic protein 4, NCC-1, Small-inducible cytokine A13, MCP-4

4 Images
Biochemical assay - Recombinant Human CCL13 Protein (Active) (AB283922)
  • Biochemical assay

Supplier Data

Biochemical assay - Recombinant Human CCL13 Protein (Active) (AB283922)

Fully biologically active determined by the dose dependent measurement of beta-arrestin recruitment on CCR3 using CHO-K1 overexpressing human CCR3/beta-arrestin/beta-galactosidase complementation system. ED50 is ≤ 181 ng/ml, corresponding to a specific activity of 5.5 x 103 units/mg.

Mass Spectrometry - Recombinant Human CCL13 Protein (Active) (AB283922)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human CCL13 Protein (Active) (AB283922)

ESI TOF analysis of ab283922.

Predicted MW is 8656.21 Da( +/- 10 Da by ESI TOF). Observed MW is 8655.74 Da.

SDS-PAGE - Recombinant Human CCL13 Protein (Active) (AB283922)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human CCL13 Protein (Active) (AB283922)

SDS-PAGE analysis of ab283922.

HPLC - Recombinant Human CCL13 Protein (Active) (AB283922)
  • HPLC

Supplier Data

HPLC - Recombinant Human CCL13 Protein (Active) (AB283922)

HPLC analysis of ab283922.

Key facts

精製度

>95% SDS-PAGE

Purity by HPLC =95%

エンドトキシンレベル

<0.005 EU/µg

発現系

HEK 293 cells

タグ

Tag free

アプリケーション

FuncS, Mass Spec, SDS-PAGE, HPLC

applications

生物活性

Yes

生物学的活性

Fully biologically active determined by the dose dependent measurement of beta-arrestin recruitment on CCR3 using CHO-K1 overexpressing human CCR3/beta-arrestin/beta-galactosidase complementation system.

ED50 is ≤ 181 ng/ml, corresponding to a specific activity of 5.5x 103 units/mg.

アクセッション番号

Q99616

アニマルフリー

Yes

キャリアフリー

No

種

Human

バッファー組成

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

style
left-column

組合せ製品

Assay Kits

AB239426

Human MCP4 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human MCP4 ELISA Kit (AB239426)

  • 0
  • 0
Primary Antibodies

AB244810

Anti-MCP4 antibody [EPR19877-62] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-MCP4 antibody [EPR19877-62] - BSA and Azide free (Capture) (AB244810)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

配列情報

[{"linker":null,"sequence":"QPDALNVPSTCCFTFSSKKISLQRLKSYVITTSRCPQKAVIFRTKLGKEICADPKEKWVQNYMKHLGRKAHTLKT","proteinLength":"Full Length","predictedMolecularWeight":"8.66 kDa","actualMolecularWeight":"8.66 kDa","aminoAcidEnd":98,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q99616","tags":[]}]
style
left-column

出荷温度及び保存条件

製品の状態
Lyophilized
出荷温度
Ambient - Can Ship with Ice
短期保存温度
Ambient
長期保存温度
Ambient
True
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MCP4 also known as CCL13 is a chemokine with a molecular mass of approximately 11 kDa. It plays a critical role in attracting immune cells to sites of inflammation. MCP4 achieves this through its interaction with G protein-coupled receptors particularly CCR2 and CCR3. The protein is primarily expressed in tissues involved in immune responses including lymph nodes spleen and bone marrow. It exhibits high expression levels at sites of allergic inflammation suggesting a specific role in these processes.
Biological function summary

MCP4 functions by mediating the recruitment of monocytes eosinophils and T lymphocytes. It is not part of a larger protein complex but rather operates as part of the chemokine signaling family. MCP4 is particularly active in coordinating responses to allergens and pathogens therefore highlighting its importance in the body's defense mechanisms. As a member of the C-C motif chemokine family it shares structural characteristics with other chemokines like CCL11 which participate in similar immune cell trafficking functions.

Pathways

MCP4 is deeply involved in inflammatory and immune pathways specifically the chemokine signaling pathway and the cytokine-cytokine receptor interaction pathway. MCP4 guides immune cells allowing them to respond effectively to cytokine signals ensuring their migration to necessary sites. Through the chemokine signaling pathway it collaborates with related chemokines like CCL5 to enhance immune responses. Its interaction with these pathways highlights its role in both routine immunity and inflammatory responses positioning it as an important player in maintaining immune homeostasis.

MCP4 correlates with conditions such as asthma and allergic rhinitis. Both of these conditions involve chronic inflammation where MCP4's role becomes more pronounced. In asthma the protein contributes to the recruitment of eosinophils and other immune cells that drive the inflammatory processes. MCP4 interactions with proteins like CCR3 are significant in exacerbating symptoms of allergic disorders making it an important target for therapeutic research aimed at modulating inflammatory responses in these diseases.
style
left-column

一般的な情報

機能

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.

配列の類似性

Belongs to the intercrine beta (chemokine CC) family.

翻訳後修飾

One major form (form long), and two minor forms (short chain and medium chain) are produced by differential signal peptide cleavage. The medium chain is about 30-fold less active than the long chain.

style
left-column

製品プロトコール

style
left-column

ターゲットの情報

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.
See full target information CCL13
style
left-column
style
left-column
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com