Recombinant human Cathepsin K protein (Active) (ab157067)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human Cathepsin K protein (Active)
Cathepsin K タンパク質・ペプチド 製品一覧 -
生理活性
≥ 1.5 U/mg protein. Activity is lot specific.
One unit is defined as the amount of enzyme that will hydrolyze 1µmol Z-Phe-Arg-AMC substrate per min. at 37°C.
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精製度
> 95 % SDS-PAGE.
ab157067 was purified as full-length proenzyme, then auto-activated at low pH. -
発現系
Baculovirus infected insect cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MWGLKVLLLP VVSFALYPEE ILDTHWELWK KTHRKQYNNK VDEISRRLIW EKNLKYISIH NLEASLGVHT YELAMNHLGD MTSEEVVQKM TGLKVPLSHS RSNDTLYIPE WEGRAPDSVD YRKKGYVTPV KNQGQCGSCW AFSSVGALEG QLKKKTGKLL NLSPQNLVDC VSENDGCGGG YMTNAFQYVQ KNRGIDSEDA YPYVGQEESC MYNPTGKAAK CRGYREIPEG NEKALKRAVA RVGPVSVAID ASLTSFQFYS KGVYYDESCN SDNLNHAVLA VGYGIQKGNK HWIIKNSWGE NWGNKGYILM ARNKNNACGI ANLASFPKM -
予測される分子量
26 kDa -
領域
1 to 329
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特性
Our Abpromise guarantee covers the use of ab157067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.41% Sodium acetate, 0.08% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.015% EDTA, 0.29% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Cathepsin K
- Cathepsin O
- Cathepsin O1
see all -
機能
Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. -
組織特異性
Predominantly expressed in osteclasts (bones). -
関連疾患
Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. -
配列類似性
Belongs to the peptidase C1 family. -
細胞内局在
Lysosome. - Information by UniProt
画像
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Functional Studies - Recombinant human Cathepsin K protein (ab157067)
Each lane contains 1 ug of total protein during time-course activation at low pH.
Lane 1: Pre-Activation.
Lane 2: 0 min
Lane 3: 1 hour
Lane 4: 2 hours
Lane 5: 3 hours
Lane 6: 4 hours
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SDS-PAGE - Recombinant human Cathepsin K protein (ab157067)ab157067 on SDS-PAGE.
Lane 1: 1 ug.
Lane 2: 2 ug.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab157067 は 3 報の論文で使用されています。
- Yoon MC et al. Molecular Features of CA-074 pH-Dependent Inhibition of Cathepsin B. Biochemistry 61:228-238 (2022). PubMed: 35119840
- Ewanchuk BW et al. A non-immunological role for ?-interferon-inducible lysosomal thiol reductase (GILT) in osteoclastic bone resorption. Sci Adv 7:N/A (2021). PubMed: 33893096
- Yoon MC et al. Selective Neutral pH Inhibitor of Cathepsin B Designed Based on Cleavage Preferences at Cytosolic and Lysosomal pH Conditions. ACS Chem Biol 16:1628-1643 (2021). PubMed: 34416110