Recombinant human Caspase-6/CASP-6 protein (ab52157)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human Caspase-6/CASP-6 protein
Caspase-6/CASP-6 タンパク質・ペプチド 製品一覧 -
生理活性
SPECIFIC ACTIVITY: 13,000 units/mg
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精製度
> 95 % SDS-PAGE. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MSSASGLRRGHPAGGEENMTETDAFYKREMFDPAEKYKMDHRRRGIALIF NHERFFWHLTLPERRGTCADRDNLTRRFSDLGFEVKCFNDLKAEELLLKI HEVSTVSHADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTGLFKGDKCHS LVGKPKIFIIQACRGNQHDVPVIPLDVVDNQTEKLDTNITEVDAASVYTL PAGADFLMCYSVAEGYYSHRETVNGSWYIQDLCEMLGKYGSSLEFTELLT LVNRKVSQRRVDFCKDPSAIGKKQVPCFASMLTKKLHFFPKSN
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab52157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
備考
This product is manufactured by BioVision, an Abcam company and was previously called 1086 Caspase-6, human recombinant. 1086-100 is the same size as the 100 unit size of ab52157.
UNIT DEFINITION: One unit of the recombinant Caspase-6 / CASP-6 is the enzyme activity that cleaves 1 nmol of the caspase substrate VEID-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: PBS, 15% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute to 1 unit per µl in PBS containing 15% glycerol. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –80°C.
関連情報
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別名
- Apoptotic protease Mch-2
- Apoptotic protease MCH2
- CASP-6
see all -
機能
Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. -
配列類似性
Belongs to the peptidase C14A family. -
翻訳後修飾
Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits. -
細胞内局在
Cytoplasm. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab52157 は 3 報の論文で使用されています。
- Hung MC et al. AKT phosphorylation as a predictive biomarker for PI3K/mTOR dual inhibition-induced proteolytic cleavage of mTOR companion proteins in small cell lung cancer. Cell Biosci 12:122 (2022). PubMed: 35918763
- Zandy AJ et al. Role of the executioner caspases during lens development. J Biol Chem 280:30263-72 (2005). PubMed: 15994297
- Basu A et al. Proteolytic activation of protein kinase C-epsilon by caspase-mediated processing and transduction of antiapoptotic signals. J Biol Chem 277:41850-6 (2002). PubMed: 12198125