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Recombinant Human Carbonic Anhydrase 9/CA9 protein (ab114200)

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SDS-PAGE - Recombinant Human Carbonic Anhydrase 9/CA9 protein (ab114200)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: ELISA, SDS-PAGE, WB

    製品の詳細

    • 製品名

      Recombinant Human Carbonic Anhydrase 9/CA9 protein
      Carbonic Anhydrase 9/CA9 タンパク質・ペプチド 製品一覧

    • 発現系

      Wheat germ

    • アクセッション番号

      Q16790

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MAPLCPSPWLPLLIPAPAPGLTVQLLLSLLLLMPVHPQRLPRMQEDSPLG GGSSGEDDPLGEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKP KSEEEGSLKLEDLPTVEAPGDPQEPQNNAHRDKEGDDQSHWRYGGDPPWP RVSPACAGRFQSPVDIRPQLAAFCPALRPLELLGFQLPPLPELRLRNNGH SVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHTVEGHRFPAEI HVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIA EEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCAQGVIWTVFNQTVM LSAKQLHTLSDTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRA AEPVQLNSCLAAGDILALVFGLLFAVTSVAFLVQMRRQHRRGTKGGVSYR PAEVAETGA

      • 予測される分子量

        77 kDa including tags

      • 領域

        1 to 459

    特性

    Our Abpromise guarantee covers the use of ab114200 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      ELISA

      SDS-PAGE

      Western blot

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    関連情報

    • 別名

      • CA-IX
      • CA9
      • CAH9_HUMAN
      • CAIX
      • Carbonate dehydratase IX
      • Carbonic anhydrase 9
      • Carbonic anhydrase IX
      • Carbonic dehydratase
      • G250
      • Membrane antigen MN
      • MN
      • P54/58N
      • pMW1
      • RCC associated protein G250
      • RCC-associated antigen G250
      • Renal cell carcinoma-associated antigen G250
      see all

    • 機能

      Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

    • 組織特異性

      Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.

    • 配列類似性

      Belongs to the alpha-carbonic anhydrase family.
      Contains 1 alpha-carbonic anhydrase domain.

    • 翻訳後修飾

      Asn-346 bears high-mannose type glycan structures.

    • 細胞内局在

      Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human Carbonic Anhydrase 9/CA9 protein (ab114200)
      SDS-PAGE - Recombinant Human Carbonic Anhydrase 9/CA9 protein (ab114200)
      12.5% SDS-PAGE showing ab114200 at approximately 76.56kDa stained with Coomassie Blue.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab114200 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab114200 は論文での使用が確認できていません。

    レビューと Q&A

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    Question

    Hello, I am writing you re your Indirect ELISA kits and hope you can advise which kit buy and protocol to use. We have developed RCC mouse model using  RENCA mouse RCC cells over expressing the human CAIX protein. We would like to test/control for the possible presence of mouse anti human CAIX antibodies in the mouse sera. That is to say we would like to coat the plates with recombinant human CAIX protein, use OUR mice sera as primary Abs (as a “detector Antibodies”) and then follow with a secondary Anti Mouse Ab  HRP Conjugated/other detection conjugate. Could you possibly advise which kit buy and protocol to use. Thank you

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    Answer

    Thank you for contacting us. Unfortunately we do not have any kit corresponding to the detection of the CAIX protein. However, I would suggest to develop an ELISA assay using the CAIX protein ab114200 that would be coated on the plate. A control anti-CAIX, such as ab15086, for which the concentration is known, may be used to create a standard curve and to quantify the amount of anti-CAIX antibodies contained in the mouse sera. ab15086 is a rabbit antibody. A mouse antibody would ideal, unfortunately, the only mouse anti-CAIX we have (ab107257) is not purified. It is an ascites surpernatant and the concentration has therefore not been determined. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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