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Recombinant Human Carbonic Anhydrase 1/CA1 protein (ab89359)

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SDS-PAGE - Recombinant Human Carbonic Anhydrase 1/CA1 protein (ab89359)
  • Western blot - Recombinant Human Carbonic Anhydrase 1/CA1 protein (ab89359)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Suitable for: WB, SDS-PAGE

製品の詳細

特性

Our Abpromise guarantee covers the use of ab89359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

    SDS-PAGE

  • 製品の状態

    Liquid

  • 備考

     This product was previously labelled as Carbonic Anhydrase I

     

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.0154% DTT, 0.316% Tris HCl, 10% Glycerol (glycerin, glycerine)

関連情報

  • 別名

    • CA 1
    • CA I
    • CA-I
    • CA1
    • CAB
    • CAH1_HUMAN
    • CAI
    • CAN
    • Car 1
    • Car1
    • Carbonate dehydratase I
    • Carbonic anhydrase 1
    • Carbonic anhydrase A
    • Carbonic anhydrase B
    • Carbonic anhydrase B, formerly
    • Carbonic anhydrase I
    • Carbonic dehydratase
    • ECK0125
    • JW0122
    • yadF
    see all

  • 機能

    Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.

  • 配列類似性

    Belongs to the alpha-carbonic anhydrase family.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

画像

  • SDS-PAGE - Recombinant Human Carbonic Anhydrase 1/CA1 protein (ab89359)
    SDS-PAGE - Recombinant Human Carbonic Anhydrase 1/CA1 protein (ab89359)

    SDS-PAGE analysis of ab89359 (3 µg) under reducing conditions and visualized by coomassie blue stain.

  • Western blot - Recombinant Human Carbonic Anhydrase 1/CA1 protein (ab89359)
    Western blot - Recombinant Human Carbonic Anhydrase 1/CA1 protein (ab89359)
    Anti-Carbonic Anhydrase 1/CA1 antibody (ab92649) at 1/500 dilution + Recombinant Human Carbonic Anhydrase 1/CA1 protein (ab89359) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 20 seconds

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab89359 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab89359 は論文での使用が確認できていません。

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