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Recombinant Human Calpain 2 protein (ab114344)

Reviews (2)Q&A (1)
SDS-PAGE - Recombinant Human Calpain 2 protein (ab114344)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: ELISA, WB, SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant Human Calpain 2 protein
      Calpain 2 タンパク質・ペプチド 製品一覧

    • 発現系

      Wheat germ

    • アクセッション番号

      P17655

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MAGIAAKLAKDREAAEGLGSHERAIKYLNQDYEALRNECLEAGTLFQDPS FPAIPSALGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQG ALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWV EVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGAT TEGFEDFTGGIAEWYELKKPPPNLFKIIQKALQKGSLLGCSIDITSAADS EAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPWGEVEWTGRWNDN CPSWNTIDPEERERLTRRHEDGEFWMSFSDFLRHYSRLEICNLTPDTLTS DTYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEED GESGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELSGQTNIHLSK NFFLTNRARERSDTFINLREVLNRFKLPPGEYILVPSTFEPNKDGDFCIR VFSEKKADYQAVDDEIEANLEEFDISEDDIDDGFRRLFAQLAGEDAEISA FELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDSDGSGKLGLKEFYIL WTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKMPCQLHQVIVARF ADDQLIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIELDLISWLCFSVL

      • 予測される分子量

        103 kDa including tags

      • 領域

        1 to 700

    関連製品

    特性

    Our Abpromise guarantee covers the use of ab114344 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      ELISA

      Western blot

      SDS-PAGE

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    関連情報

    • 別名

      • Calcium activated neutral proteinase
      • Calcium activated neutral proteinase 2
      • Calcium-activated neutral protease 2, catalytic subunit
      • Calcium-activated neutral proteinase 2
      • CALP80
      • Calpain 2 (m/II) large subunit
      • Calpain 2 catalytic subunit
      • Calpain 2 large catalytic subunit
      • Calpain 2 large subunit
      • Calpain 2, large [catalytic] subunit
      • Calpain large polypeptide L2
      • Calpain M type
      • Calpain M-type
      • Calpain-2 catalytic subunit
      • Calpain-2 large subunit
      • Calpain2
      • CAN2_HUMAN
      • CANP 2
      • CANP L2
      • CANP2
      • CANPL 2
      • CANPL2
      • CANPml
      • Capa2
      • CAPN 2
      • CAPN2
      • FLJ39928
      • M calpain
      • M calpin
      • M type
      • M-calpain
      • mCANP
      • Millimolar calpain
      • Millimolar-calpain
      see all

    • 機能

      Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

    • 組織特異性

      Ubiquitous.

    • 配列類似性

      Belongs to the peptidase C2 family.
      Contains 1 calpain catalytic domain.
      Contains 3 EF-hand domains.

    • 細胞内局在

      Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human Calpain 2 protein (ab114344)
      SDS-PAGE - Recombinant Human Calpain 2 protein (ab114344)
      ab114344 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab114344 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab114344 は論文での使用が確認できていません。

    レビューと Q&A

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    1-3 of 3 Abreviews or Q&A

    Functional assay on the calpain2 activity

     Poor
    Abreviews
    Application
    Functional Studies
    We ordered and tried the Recombinant Human Calpain 2 protein (ab114344) for the functional assay on its activity using calpain activity kit (ab65308, Abcam, our order number for this kit #3561741), and use the calpain1 in the kit (ab65308) as positive control. There was a very good result for the calpain1 positive control as described in the manual. However, there was the similar RFU value of calpain2 product (ab114344) with the negative control, which means the calpain2 protein product (ab114344) is not functional and is not suitable for the calpain2 functional study.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Xuefei Tian

    Verified customer

    投稿 Dec 24 2019

    Functional study testing on the calpain2 protein product

     Poor
    Abreviews
    abreview image
    Application
    Other - Do not use
    We tried the Recombinant Human Calpain2 protein (ab114344) for the functional studies on its activity using calpain activity kit (ab65308, Abcam), along with the calpain1 in the kit as positive control and without calpain as the negative control. There was a very good result for the calpain1 positive control as described in the manual. However, there was no bioactivity of the calpain 2 protein product detected. It suggests that the calpain2 protein product (ab114344) is not suitable for the functional study purpose.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Nov 22 2019

    Question

    Dear technical team,

    Our customer is looking for Calpain 2 protein with active form.

    https://www.abcam.com/Calpain-2-protein-ab114344.html

    If it is not an active form, she needs to treat calcium to make this proteinactive form but,

    calcium binding site is N-terminal and she isworried about itsN-terminal had tagged.

    Let me know if ab114344 is active form or not and tagging has possibility to inhibit calcium binding.

    Bset regards,

    Read More
    Answer

    Thank you for contacting us.

    Unfortunately we have not tested Calpain 2 protein (ab114344) for its activity, and we have had no reports of other customers doing so either. I can therefore not guarantee that the protein will retain activity in biological systems.

    As your customer has noticed, the protein is N-terminally fused with a purification tag. This could potentially affect the activity of the protein. If your customer would like to try this protein for its activity they would be eligible for a testing discount. More information on this scheme can be found from the following link:

    https://www.abcam.com/collaboratordiscount

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

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