Recombinant Human c-Myc protein (ab84132)
Key features and details
- Expression system: Escherichia coli
- Purity: > 75% Affinity purified
- Tags: His tag C-Terminus
- Suitable for: WB, SDS-PAGE
製品の詳細
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製品名
Recombinant Human c-Myc protein
c-Myc タンパク質・ペプチド 製品一覧 -
精製度
> 75 % Affinity purified.
Purified by an affinity column in combination with FPLC chromatography. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MPLNVSFTNRNYDLDYDSVQPYFYCDEEE NFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTP FSLRGDND GGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSG FSAAAKLV SEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVF PYPLNDSS SPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSE EEQEDEEE IDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAA PPSTRKDY PAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNE LKRSFFAL RDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQL KHKLEQLR NSCA -
予測される分子量
50 kDa -
領域
1 to 439 -
タグ
His tag C-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab84132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.9
Constituents: Potassium chloride, 0.0154% DTT, Tris HCl, EDTA, 20% Glycerol
Protein is stored in 20 mM Tris-Cl (pH 7.5), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA.
関連情報
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別名
- AU016757
- Avian myelocytomatosis viral oncogene homolog
- bHLHe39
see all -
機能
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. -
関連疾患
Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻訳後修飾
Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. -
細胞内局在
Nucleus > nucleoplasm. Nucleus > nucleolus. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human c-Myc protein (ab84132)
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Western blot - Recombinant Human c-Myc protein (ab84132)SDS-PAGE with Coomassie Blue staining (Left) and Western-blot (Right)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab84132 は 7 報の論文で使用されています。
- Duan X et al. Moderate DNA hypomethylation suppresses intestinal tumorigenesis by promoting caspase-3 expression and apoptosis. Oncogenesis 10:38 (2021). PubMed: 33947834
- Morel M et al. The F-box protein FBXL16 up-regulates the stability of C-MYC oncoprotein by antagonizing the activity of the F-box protein FBW7. J Biol Chem 295:7970-7980 (2020). PubMed: 32345600
- Koncar RF et al. Identification of Novel RAS Signaling Therapeutic Vulnerabilities in Diffuse Intrinsic Pontine Gliomas. Cancer Res 79:4026-4041 (2019). PubMed: 31201162
- Favia A et al. The Protein Arginine Methyltransferases 1 and 5 affect Myc properties in glioblastoma stem cells. Sci Rep 9:15925 (2019). PubMed: 31685892
- Wang Z et al. lncRNA Epigenetic Landscape Analysis Identifies EPIC1 as an Oncogenic lncRNA that Interacts with MYC and Promotes Cell-Cycle Progression in Cancer. Cancer Cell 33:706-720.e9 (2018). PubMed: 29622465
- Felsenstein KM et al. Small Molecule Microarrays Enable the Identification of a Selective, Quadruplex-Binding Inhibitor of MYC Expression. ACS Chem Biol 11:139-48 (2016). PubMed: 26462961
- Tremblay R et al. The development of a high-yield recombinant protein bioreactor through RNAi induced knockdown of ATP/ADP transporter in Solanum tuberosum. J Biotechnol 156:59-66 (2011). PubMed: 21864587