Recombinant human Brk/PTK6 protein (ab60888)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human Brk/PTK6 protein
Brk/PTK6 タンパク質・ペプチド 製品一覧 -
精製度
> 90 % SDS-PAGE.
Purity: >90% as determined by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human
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関連製品
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Substrate reagent
特性
Our Abpromise guarantee covers the use of ab60888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as Brk
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- Breast tumor kinase
- Breast tumour kinase
- Protein tyrosine kinase 6
see all -
機能
Phosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression. -
組織特異性
Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻訳後修飾
Autophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme. -
細胞内局在
Cytoplasm. Nucleus. Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors. - Information by UniProt
画像
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Functional Studies - Recombinant human Brk/PTK6 protein (ab60888)The specific activity of Brk/PTK6 (ab60888) was determined to be 136 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human Brk/PTK6 protein (ab60888)SDS PAGE analysis of ab60888 -
SDS-PAGE - Recombinant human Brk/PTK6 protein (ab60888)SDS PAGE analysis of ab60888 -
Functional Studies - Recombinant human Brk/PTK6 protein (ab60888)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant human Brk/PTK6 protein (ab60888)ab60888 on SDS-PAGE, MW ~80 kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab60888 は 1 報の論文で使用されています。
- Miczi M et al. Identification of Host Cellular Protein Substrates of SARS-COV-2 Main Protease. Int J Mol Sci 21:N/A (2020). PubMed: 33333742