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AB204208

Recombinant human BRAF protein

Recombinant human BRAF protein

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(1 Publication)

Recombinant human BRAF protein is a Human Fragment protein, in the 381 to 766 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

別名を表示する

BRAF1, RAFB1, BRAF, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1

2 Images
Functional Studies - Recombinant human BRAF protein (AB204208)
  • FuncS

Supplier Data

Functional Studies - Recombinant human BRAF protein (AB204208)

Kinase assay showing the specific activity of ab204208 as 2,300 nmol/min/mg.

SDS-PAGE - Recombinant human BRAF protein (AB204208)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human BRAF protein (AB204208)

The purity of ab204208 was determined to be >90% by densitometry.
Approximate MWt : 69 kDa.

Key facts

精製度

>90% Densitometry

Purity is lot specific. Please contact our technical Support team for details. Affinity purified.

発現系

Baculovirus infected Sf9 cells

タグ

GST tag N-Terminus

アプリケーション

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

The specific activity of ab204208 was determined to be 2,300 nmol/min/mg.

アクセッション番号

P15056

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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組合せ製品

Reagents, Controls & Accessories

AB325996

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 12 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 12 bands) (AB325996)

  • 0
  • 0
Primary Antibodies

AB228461

Anti-BRAF (mutated V600E) antibody [VE1]

20ul selling size|BOND RX™ Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF (mutated V600E) antibody [VE1] (AB228461)

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  • 7
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB313357

Recombinant Human NRAS Protein (His tag) (E.Coli)

Mass Spectrometry - Recombinant Human NRAS Protein (His tag) (E.Coli) (AB313357)

  • 0
  • 0
Proteins & Peptides

AB60755

Recombinant mouse PDGFR alpha protein

Functional Studies - Recombinant mouse PDGFR alpha protein (AB60755)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"DLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH","proteinLength":"Fragment","predictedMolecularWeight":"69 kDa","actualMolecularWeight":null,"aminoAcidEnd":766,"aminoAcidStart":381,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P15056","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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一般的な情報

機能

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed : 21441910, PubMed : 29433126). Phosphorylates PFKFB2 (PubMed : 36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed : 1508179).

配列の類似性

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

翻訳後修飾

Phosphorylation at Ser-365 by SGK1 inhibits its activity (Ref.8, PubMed:11410590). Phosphorylation at Thr-753 by MAPK1 (PubMed:19710016). Dephosphorylation of Ser-365 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC); this relieves inactivation and stimulates kinase activity (PubMed:35768504, PubMed:35830882).. Methylation at Arg-671 decreases stability and kinase activity.. Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.

細胞内局在性

Nucleus

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製品プロトコール

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ターゲットの情報

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed : 21441910, PubMed : 29433126). Phosphorylates PFKFB2 (PubMed : 36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed : 1508179).
See full target information BRAF
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 185:1974-1985.e12 PubMed35512704

2022

Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiulei Mo,Qiankun Niu,Andrey A Ivanov,Yiu Huen Tsang,Cong Tang,Changfa Shu,Qianjin Li,Kun Qian,Alafate Wahafu,Sean P Doyle,Danielle Cicka,Xuan Yang,Dacheng Fan,Matthew A Reyna,Lee A D Cooper,Carlos S Moreno,Wei Zhou,Taofeek K Owonikoko,Sagar Lonial,Fadlo R Khuri,Yuhong Du,Suresh S Ramalingam,Gordon B Mills,Haian Fu
View all publications
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Abcam product promise

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