Recombinant human BMP2 protein (Active) (ab50099)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: HPLC, Cellular Activation, SDS-PAGE
製品の詳細
-
製品名
Recombinant human BMP2 protein (Active)
BMP2 タンパク質・ペプチド 製品一覧 -
生理活性
Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells.
The expected ED50 for this effect is 0.5-1.0 μg/ml.
-
精製度
> 98 % SDS-PAGE.
>98% by HPLC analyses. -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR -
予測される分子量
26 kDa -
領域
283 to 396 -
配列の追加情報
Homodimeric protein consisting of two 115 amino acid polypeptide chains. Full length mature protein, minus the signal peptide and the propeptide.
-
特性
Our Abpromise guarantee covers the use of ab50099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
HPLC
Cellular Activation
SDS-PAGE
-
製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
再構成Centrifuge the vial prior to opening. Reconstitute in water containing BSA (50ug of BSA per 1ug of protein) to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
関連情報
-
別名
- BDA2
- BMP-2
- BMP-2A
see all -
機能
Induces cartilage and bone formation. -
組織特異性
Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine. -
配列類似性
Belongs to the TGF-beta family. -
細胞内局在
Secreted. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (2)
ab50099 は 2 報の論文で使用されています。
- Zhang W et al. Preparation of a nanopearl powder/C-HA (chitosan-hyaluronic acid)/rhBMP-2 (recombinant human bone morphogenetic protein-2) composite artificial bone material and a preliminary study of its effects on MC3T3-E1 cells. Bioengineered 13:14368-14381 (2022). PubMed: 35758269
- Xie YF et al. Pulsed electromagnetic fields stimulate osteogenic differentiation and maturation of osteoblasts by upregulating the expression of BMPRII localized at the base of primary cilium. Bone 93:22-32 (2016). PubMed: 27622883