Recombinant human BACE1 protein (Active) (ab168075)
Key features and details
- Expression system: Insect cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human BACE1 protein (Active)
BACE1 タンパク質・ペプチド 製品一覧 -
生理活性
Determined using a ß-Secretase Activity Assay Kit. Specific units > 120x103 units/mg. One unit is defined as the amount of enzyme that produces 1 pmol/min EDANS by hydrolyzing the substrate BVT9964 (NH2-Arg-Glu(EDANS)-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)-Arg-COOH) at 37°C in 100 mM acetate buffer pH 4.0.
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精製度
> 90 % SDS-PAGE. -
発現系
Insect cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
予測される分子量
47 kDa -
領域
46 to 460 -
配列の追加情報
Mature protease domain.
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特性
Our Abpromise guarantee covers the use of ab168075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
備考
This product is manufactured by BioVision, an Abcam company and was previously called 7609 Active Recombinant Human beta-Secretase 1 - BACE-1. 7609-5 is the same size as the 5 µg size of ab168075.
Produced using non-baculovirus insect cells.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 50% Glycerol (glycerin, glycerine), PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- APP beta secretase
- Asp 2
- ASP2
see all -
機能
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. -
組織特異性
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. -
配列類似性
Belongs to the peptidase A1 family. -
ドメイン
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. -
翻訳後修飾
Glycosylated. -
細胞内局在
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. - Information by UniProt
画像
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Functional Studies - Recombinant human BACE1 protein (Active) (ab168075)
Example of BACE1 Activity Assay.
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SDS-PAGE - Recombinant human BACE1 protein (Active) (ab168075)SDS-PAGE analysis of ab168075.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab168075 は論文での使用が確認できていません。