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AB64298

Recombinant ヒト Axl protein (His tag N-Terminus)

Recombinant human Axl protein (His tag N-Terminus)

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Recombinant human Axl protein (His tag N-Terminus) is a Human Fragment protein, in the 473 to 894 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

別名を表示する

UFO, AXL, Tyrosine-protein kinase receptor UFO, AXL oncogene

4 Images
Functional Studies - Recombinant human Axl protein (His tag N-Terminus) (AB64298)
  • FuncS

Unknown

Functional Studies - Recombinant human Axl protein (His tag N-Terminus) (AB64298)
Functional Studies - Recombinant human Axl protein (His tag N-Terminus) (AB64298)
  • FuncS

Unknown

Functional Studies - Recombinant human Axl protein (His tag N-Terminus) (AB64298)

The specific activity of Axl (ab64298) was determined to be 88 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human Axl protein (His tag N-Terminus) (AB64298)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Axl protein (His tag N-Terminus) (AB64298)

SDS PAGE analysis of ab64298

SDS-PAGE - Recombinant human Axl protein (His tag N-Terminus) (AB64298)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Axl protein (His tag N-Terminus) (AB64298)

SDS Page analysis of ab64298.

Key facts

精製度

>90% SDS-PAGE

Affinity purified.

発現系

Baculovirus infected Sf9 cells

タグ

His tag N-Terminus

アプリケーション

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

Specific activity: 104 nmol/min/mg.

アクセッション番号

P30530

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.00308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

storage-buffer

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組合せ製品

Sample Prep & Detection Kits

AB201796

Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link®

Conjugation - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type B) - Lightning-Link® (AB201796)

  • 3
  • 1
Primary Antibodies

AB89224

Anti-Axl antibody [MM0098-2N33]

Immunocytochemistry/ Immunofluorescence - Anti-Axl antibody [MM0098-2N33] (AB89224)

  • 5
  • 4
Multiplex Kits

AB133995

Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 62 Targets)

Multiplex Protein Detection - Cytokine Array - Mouse Cytokine Antibody Array (Membrane, 62 Targets) (AB133995)

  • 0
  • 0
Assay Kits

AB208987

Human Axl ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Axl ELISA Kit (AB208987)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Kinase assay.</p>" } } }
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製品の詳細

ab204853 (IRS1 peptide) can be utilized as a substrate for assessing kinase activity
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配列情報

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":894,"aminoAcidStart":473,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P30530","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Axl protein also known as AXL receptor tyrosine kinase plays a significant role in cell signaling. It has a molecular weight of approximately 98 kDa. Axl is a transmembrane receptor that is expressed in a variety of tissues including the immune system reproductive organs and the central nervous system. It is mainly recognized for transmitting signals from the extracellular matrix into the cytoplasm by binding with its ligand Gas6.
Biological function summary

Axl is important in mediating cell survival proliferation and migration. It functions as part of the TAM family of receptors which also includes Tyro3 and Mer. Axl often forms complexes with these receptors to facilitate efficient signaling. This interaction triggers phosphorylation events that activate downstream signaling proteins reinforcing its influence in cellular functions.

Pathways

Axl is deeply involved in the PI3K-Akt and MAPK signaling pathways which are central to cellular growth and survival. In these pathways Axl interacts closely with proteins like PI3K and Akt to regulate processes such as apoptosis and metabolism. Its interaction with these pathways places Axl as an important modulator of intracellular signaling cascades.

Axl has been implicated in cancer progression and immune response dysregulation. In many cancer types Axl overexpression links to tumor invasion metastasis and resistance to therapy. Moreover Axl’s role in autoimmune diseases becomes evident through its association with the Gas6 protein. Exploring these connections furthers understanding of how Axl's aberrant activity influences health and disease.
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一般的な情報

機能

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.. (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.. (Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.. (Microbial infection) Promotes Zika virus entry in glial cells, Sertoli cells and astrocytes (PubMed : 28076778, PubMed : 29379210, PubMed : 31311882). Additionally, Zika virus potentiates AXL kinase activity to antagonize type I interferon signaling and thereby promotes infection (PubMed : 28076778). Interferon signaling inhibition occurs via an SOCS1-dependent mechanism (PubMed : 29379210).

配列の類似性

Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.

翻訳後修飾

Monoubiquitinated upon GAS6-binding. A very small proportion of the receptor could be subjected to polyubiquitination in a very transient fashion.. Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity.

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製品プロトコール

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ターゲットの情報

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Also plays an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.. (Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.. (Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.. (Microbial infection) Promotes Zika virus entry in glial cells, Sertoli cells and astrocytes (PubMed : 28076778, PubMed : 29379210, PubMed : 31311882). Additionally, Zika virus potentiates AXL kinase activity to antagonize type I interferon signaling and thereby promotes infection (PubMed : 28076778). Interferon signaling inhibition occurs via an SOCS1-dependent mechanism (PubMed : 29379210).
See full target information AXL
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