Recombinant Human ASF1b protein (ab130033)
Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, MS
製品の詳細
-
製品名
Recombinant Human ASF1b protein -
精製度
> 85 % SDS-PAGE.
ab130033 was purified by using conventional chromatography techniques. -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
配列
MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH INWDNNMDRL EAIETQDPSL GCGLPLNCTP IKGLGLPGCI PGLLPENSMD CILEHHHHHH -
予測される分子量
23 kDa including tags -
領域
1 to 202 -
タグ
His tag C-Terminus
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab130033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
Mass Spectrometry
-
質量分析
MALDI-TOF -
製品の状態
Liquid -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.88% Sodium chloride, 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% DTT
関連情報
-
別名
- Anti silencing function 1B
- Anti silencing function 1B histone chaperone
- Anti-silencing function protein 1 homolog B
see all -
機能
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis. -
組織特異性
Highly expressed in testis and at lower levels in colon, small intestine and thymus. -
配列類似性
Belongs to the ASF1 family. -
翻訳後修飾
Phosphorylated by TLK1 and TLK2. -
細胞内局在
Nucleus. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab130033 は 1 報の論文で使用されています。
- Carraro M et al. DAXX adds a de novo H3.3K9me3 deposition pathway to the histone chaperone network. Mol Cell 83:1075-1092.e9 (2023). PubMed: 36868228