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Recombinant Human APPBP1 protein (ab116199)

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SDS-PAGE - Recombinant Human APPBP1 protein (ab116199)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant Human APPBP1 protein

    • 精製度

      > 90 % SDS-PAGE.
      ab116199 was purified using conventional chromatography techniques.

    • 発現系

      Escherichia coli

    • アクセッション番号

      Q13564

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MGSSHHHHHHSSGLVPRGSHMGSMAQLGKLLKEQKYDRQLRLWGDHGQEA LESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQ RSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVV ATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPD NALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYS ETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALN TTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRG TIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISE KELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYL MLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDD YVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQ TSATFQL

      • 予測される分子量

        63 kDa including tags

      • 領域

        1 to 534

      • タグ

        His tag N-Terminus

    特性

    Our Abpromise guarantee covers the use of ab116199 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      SDS-PAGE

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.03% DTT, 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 1.17% Sodium chloride

    関連情報

    • 別名

      • 59 kDa
      • A 116A10.1
      • Amyloid beta precursor protein binding protein 1
      • Amyloid beta precursor protein binding protein 1 59kD
      • Amyloid beta precursor protein binding protein 1 59kDa
      • Amyloid beta precursor protein binding protein 1, 59 kDa
      • Amyloid beta precursor protein-binding protein 1
      • Amyloid protein binding protein 1
      • Amyloid protein-binding protein 1
      • APP BP1
      • APP-BP1
      • APPBP 1
      • APPBP1
      • HPP 1
      • HPP1
      • NAE 1
      • NAE1
      • NEDD8 activating enzyme E1 regulatory subunit
      • NEDD8 activating enzyme E1 subunit
      • NEDD8 activating enzyme E1 subunit 1
      • NEDD8-activating enzyme E1 regulatory subunit
      • Proto oncogene protein 1
      • Proto-oncogene protein 1
      • Protooncogene protein 1
      • ula 1
      • ULA1_HUMAN
      see all

    • 機能

      Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.

    • 組織特異性

      Ubiquitous in fetal tissues. Expressed throughout the adult brain.

    • パスウェイ

      Protein modification; protein neddylation.

    • 配列類似性

      Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.

    • 細胞内局在

      Cell membrane. Colocalizes with APP in lipid rafts.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human APPBP1 protein (ab116199)
      SDS-PAGE - Recombinant Human APPBP1 protein (ab116199)
      15% SDS-PAGE analysis of 3 µg ab116199.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab116199 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab116199 は論文での使用が確認できていません。

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