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Recombinant Human Apolipoprotein B (ab80037)

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SDS-PAGE - Recombinant Human Apolipoprotein B (ab80037)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 80% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: WB, ELISA, SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant Human Apolipoprotein B
      Apolipoprotein B タンパク質・ペプチド 製品一覧

    • 精製度

      > 80 % SDS-PAGE.

    • 発現系

      Escherichia coli

    • アクセッション番号

      P04114

    • タンパク質長

      Protein fragment

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        NPVSKGLLIFDASSSWGPQMSASVHLDSKKKQHLFVKEVKIDGQFRVSSF YAKGTYGLSCQRDPNTGRLNGESNLRFNSSYLQGTNQITGRYEDGTLSLT STSDLQSGIIKNTASLKYENYELTLKSDTNGKYKNFATSNKMDMTFSKQN ALLRSEYQADY

      • 予測される分子量

        31 kDa including tags

      • 領域

        1406 to 1606

      • タグ

        His tag N-Terminus

      • 配列の追加情報

        E. coli derived ApoB100 fusion protein with His tag

    特性

    Our Abpromise guarantee covers the use of ab80037 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Western blot

      ELISA

      SDS-PAGE

    • 製品の状態

      Lyophilized
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      Constituent: PBS

    • 再構成
      Redissolve the powder with 1 ml sterile water will restore to the original concentration 1 mg/ml.

    関連情報

    • 別名

      • Apo B
      • Apo B 100
      • Apo B-100
      • Apo B-48
      • Apo B100
      • Apo B48
      • APOB
      • ApoB 100
      • ApoB 48
      • APOB_HUMAN
      • Apolipoprotein B
      • Apolipoprotein B (including Ag(x) antigen)
      • Apolipoprotein B 100
      • Apolipoprotein B 48
      • Apolipoprotein B-48
      • Apolipoprotein B100
      • Apolipoprotein B48
      • FLDB
      • LDLCQ4
      see all

    • 機能

      Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

    • 関連疾患

      Hypobetalipoproteinemia, familial, 1
      Familial ligand-defective apolipoprotein B-100
      Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

    • 配列類似性

      Contains 1 vitellogenin domain.

    • 翻訳後修飾

      Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.

    • 細胞内局在

      Cytoplasm. Secreted.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human Apolipoprotein B (ab80037)
      SDS-PAGE - Recombinant Human Apolipoprotein B (ab80037)
      20% SDS-PAGE gel analysis showing ab80037 at approximately 31 kDa. Commassie blue staining.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab80037 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab80037 は論文での使用が確認できていません。

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    Protein is cloudy and there is precipitate in the vial. What is in this solution? Other proteins? Are there suggestions for getting it into solution?

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    Answer

    The protein was lyophilized from 1x PBS and there is no BSA or other protein in the buffer. If the solution is cloudy we don't expect there to be an issue with the quality, however if there is precipitate we suggest adding more water and agitating the solution to help it solubilize. Do not add other materials to the solution to aid reconstitution.

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    Question

    Are there any antibodies that are specific for the region of apolipoprotein B that is protein ab80037?

    Read More
    Answer

    We have heard back from the lab regarding your inquiry about an ApoB antibody specific to the region from which the peptide ab80037 has been created. Unfortunately, the lab has not produced an antibody agaist this antigen. In review of our catalog, while we do offer a number of ApoB antibodies these have been created either from the full length protein or by peptides from different regions.

    Read More

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