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AB113861

Recombinant Human Apolipoprotein A V/APOA5

Recombinant Human Apolipoprotein A V/APOA5

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(1 Publication)

Recombinant Human Apolipoprotein A V/APOA5 is a Human Full Length protein, in the 24 to 366 aa range, expressed in HEK 293 cells, with >70%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, ELISA, WB.

別名を表示する

RAP3, UNQ411/PRO773, APOA5, Apolipoprotein A-V, Apo-AV, ApoA-V, Apolipoprotein A5, Regeneration-associated protein 3

1 Images
SDS-PAGE - Recombinant Human Apolipoprotein A V/APOA5 (AB113861)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Apolipoprotein A V/APOA5 (AB113861)

12% SDS-PAGE separation of Human Apolipoprotein A V/APOA5
1.reduced and boiled sample, 5μg/lane
2.non-reduced and non-boiled sample, 5μg/lane

Key facts

精製度

>70% SDS-PAGE

Purity determined by densitometric image analysis

エンドトキシンレベル

< 1 EU/µg

発現系

HEK 293 cells

タグ

DDDDK tag C-Terminus

アプリケーション

SDS-PAGE, ELISA, WB

applications

生物活性

No

アクセッション番号

Q6Q788

アニマルフリー

No

キャリアフリー

No

種

Human

再構成

Reconstitute in 200 µL of water

バッファー組成

Constituents: 0.29% Sodium chloride, 0.24% Tris

storage-buffer

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組合せ製品

Proteins & Peptides

AB322097

Recombinant Mouse APOA1 Protein Standard (His tag)

Sandwich ELISA - Recombinant Mouse APOA1 Protein Standard (His tag) (AB322097)

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  • 0
Proteins & Peptides

AB226264

Recombinant Mouse Apolipoprotein CIII (Tagged)

SDS-PAGE - Recombinant Mouse Apolipoprotein CIII (Tagged) (AB226264)

  • 0
  • 0
Proteins & Peptides

AB280330

Recombinant human Apolipoprotein E3

Mass Spectrometry - Recombinant human Apolipoprotein E3 (AB280330)

  • 0
  • 0
Proteins & Peptides

AB114408

Recombinant Human CETP protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human CETP protein (GST tag N-Terminus) (AB114408)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

This product was previously labelled as Apolipoprotein A V
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配列情報

[{"linker":null,"sequence":"RKGFWDYFSQTSGDKGRVEQIHQQKMAREPATLKDSLEQDLNNMNKFLEKLRPLSGSEAPRLPQDPVGMRRQLQEELEEVKARLQPYMAEAHELVGWNLEGLRQQLKPYTMDLMEQVALRVQELQEQLRVVGEDTKAQLLGGVDEAWALLQGLQSRVVHHTGRFKELFHPYAESLVSGIGRHVQELHRSVAPHAPASPARLSRCVQVLSRKLTLKAKALHARIQQNLDQLREELSRAFAGTGTEEGAGPDPQMLSEEVRQRLQAFRQDTYLQIAAFTRAIDQETEEVQQQLAPPPPGHSAFAPEFQQTDSGKVLSKLQARLDDLWEDITHSLHDQGHSHLGDPAAADYKDDDDK","proteinLength":"Full Length","predictedMolecularWeight":"40.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":366,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q6Q788","tags":[{"tag":"DDDDK","terminus":"C-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Lyophilized
出荷温度
Blue Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A V also known as APOA5 plays a critical role in lipid metabolism. This protein possesses a molecular weight of approximately 39 kDa and is mainly expressed in the liver. APOA5 modulates the plasma triglyceride levels by enhancing the uptake and clearance of triglyceride-rich lipoproteins. The protein is located at the surface of these lipoproteins facilitating their interaction with receptors and enzymes like lipoprotein lipase.
Biological function summary

APOA5 influences triglyceride metabolism and is part of the HDL particle complex. It interacts directly with lipoprotein lipase to promote the hydrolysis of triglycerides into free fatty acids. This hydrolytic action aids in reducing triglyceride levels in the plasma. APOA5 also indirectly influences very-low-density lipoprotein (VLDL) metabolism reinforcing its role in lipid regulation.

Pathways

APOA5 integrates into the lipoprotein metabolic pathway and the PPAR signaling pathway. In the lipoprotein metabolic pathway APOA5 works with proteins like APOC3 to influence triglyceride homeostasis. This protein is modulated by PPAR-alpha helping control lipid metabolism and energy homeostasis. Its role in these pathways highlights its impact on triglyceride-rich lipoprotein metabolism positioning it as an important player in maintaining lipid balance.

APOA5 strongly associates with hypertriglyceridemia and cardiovascular disease. Variations in the APOA5 gene can lead to abnormal plasma triglyceride levels contributing to the development of hypertriglyceridemia. Additionally APOA5's connection with APOE proteins through lipid metabolism pathways can influence susceptibility to cardiovascular disorders. Understanding the interactions and mutations within APOA5 provides insights into the mechanisms contributing to these disease states.
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一般的な情報

機能

Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and an inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity). Activates poorly lecithin : cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Binds heparin (PubMed : 17326667).

配列の類似性

Belongs to the apolipoprotein A1/A4/E family.

翻訳後修飾

Phosphorylated by FAM20C in the extracellular medium.

細胞内局在性

Early endosome

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製品プロトコール

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ターゲットの情報

Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and an inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate) (By similarity). Activates poorly lecithin : cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Binds heparin (PubMed : 17326667).
See full target information APOA5
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Immunity 56:2523-2541.e8 PubMed37924812

2023

Gasdermin D permeabilization of mitochondrial inner and outer membranes accelerates and enhances pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Miao,Cong Jiang,Winston Y Chang,Haiwei Zhang,Jinsu An,Felicia Ho,Pengcheng Chen,Han Zhang,Caroline Junqueira,Dulguun Amgalan,Felix G Liang,Junbing Zhang,Charles L Evavold,Iva Hafner-Bratkovič,Zhibin Zhang,Pietro Fontana,Shiyu Xia,Markus Waldeck-Weiermair,Youdong Pan,Thomas Michel,Liron Bar-Peled,Hao Wu,Jonathan C Kagan,Richard N Kitsis,Peng Zhang,Xing Liu,Judy Lieberman
View all publications
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