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Recombinant human Angiopoietin 1 protein (ab69492)

Submit a review Q&A (2)References (1)

Key features and details

  • Expression system: HeLa cells
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, Functional Studies

製品の詳細

  • 製品名

    Recombinant human Angiopoietin 1 protein
    Angiopoietin 1 タンパク質・ペプチド 製品一覧

  • 生理活性

    Determined by its ability to induce adhesion of human umbilical vein endothelial cells (HUVEC).

  • 精製度

    > 95 % SDS-PAGE.
    Greater than 95% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).

  • 発現系

    HeLa cells

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 配列

      Sequencing analysis shows N-terminal sequences starting with Ser-20 and with Asp-70 of the 498 amino acid precursor protein.

    • タグ

      His tag C-Terminus

関連製品

特性

Our Abpromise guarantee covers the use of ab69492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 製品の状態

    Lyophilized
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Constituents: 5% Trehalose, 0.328% Sodium phosphate, 1.16% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    Reconstitution should be done in water to a concentration of 0.1-1.0mg/ml.

関連情報

  • 別名

    • AGP 1
    • AGP1
    • AGPT
    • ANG
    • ANG 1
    • ANG-1
    • ANG1
    • Angiopoietin-1
    • Angiopoietin1
    • ANGP1_HUMAN
    • ANGPT 1
    • Angpt1
    • KIAA0003
    • OTTHUMP00000227592
    • OTTHUMP00000227599
    • OTTHUMP00000227600
    see all

  • 機能

    Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.

  • 配列類似性

    Contains 1 fibrinogen C-terminal domain.

  • 翻訳後修飾

    Glycosylated.

  • 細胞内局在

    Secreted.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab69492 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab69492 は 1 報の論文で使用されています。

  • Hultström M  et al. Angiopoietin-2 Inhibition of Thrombomodulin-Mediated Anticoagulation-A Novel Mechanism That May Contribute to Hypercoagulation in Critically Ill COVID-19 Patients. Biomedicines 10:N/A (2022). PubMed: 35740360

レビューと Q&A

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レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Question

send sequence alignment between human and mosue angiopoietin 1
send IHC-P protocol

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Answer

Thank you for contacting us.

I have attached the results of the sequence alignment between mouse and human angiopoietin 1.

The link to our IHC-P protocol is as follows:
https://www.abcam.com/index.html?pageconfig=resource&rid=11384

The file is also attached for your convenience.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Has the activity of Angiopoietin 1 protein (ab69492) been tried in rats? Thanks.

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Answer

Thank you for your inquiry. I can confirm that ab69492 is an active human Angiopoietin 1 protein that showed biological activity determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC). The activity of this protein was not tested with rat derived cells and I cannot speculate about the outcome of such an experiment. But I would like to let you know that the homology between human and rat Angiopoietin 1 is 96%. I am sorry I did not have a definitive answer for you on this occasion and hope this information was nevertheless helpful.

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