Recombinant human AMPK alpha 2 + beta 1 + gamma 2 protein (ab185258)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, proprietary tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human AMPK alpha 2 + beta 1 + gamma 2 protein -
生理活性
The specific activity of ab185258 was determined to be 350 nmol/min/mg.
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精製度
> 90 % SDS-PAGE.
Assessed by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
タグ
His tag C-Terminus , proprietary tag N-Terminus -
配列の追加情報
Recombinant full length AMPK alpha 2 (aa 1-552; UniProt P54646) + AMPK beta 1 (aa 1-270; UniProt Q9Y478) + AMPK gamma 2 Isoform C (aa 1-525; UniProt Q9UGJ0-3). MWt 92, 65, and 105 kDa respectively.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab185258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
備考
AMPK alpha 2 (amino acids: 1-552; UniProt P54646)
MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAVKILNRQKIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGELFDYICKHGRVEEMEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR
AMPK beta 1 (amino acids 1-270; UniProt Q9Y478)
MGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYVCKPEERFRAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI
AMPK gamma 2 Isoform C (amino acids 1-525; UniProt Q9UGJ0-3)
MPLLDGDLEGSGKHSSRKVDSPFGPGSPSKGFFSRGPQPRPSSPMSAPVRPKTSPGSPKTVFPFSYQESPPRSPRRMSFSGIFRSSSKESSPNSNPATSPGGIRFFSRSRKTSGLSSSPSTPTQVTKQHTFPLESYKHEPERLENRIYASSSPPDTGQRFCPSSFQSPTRPPLASPTHYAPSKAAALAAALGPAEAGMLEKLEFEDEAVEDSESGVYMRFMRSHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWESKKQSFVGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRELYLQETFKPLVNISPDASLFDAVYSLIKNKIHRLPVIDPISGNALYILTHKRILKFLQLFMSDMPKPAFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKCNKLEILETIVDRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPAGAKQKETETEab204881 (Acetyl Coenzyme A Carboxylase peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7
Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- 5' AMP activated protein kinase beta 1 subunit
- 5' AMP activated protein kinase catalytic alpha 2 chain
- 5' AMP activated protein kinase catalytic subunit alpha 2
see all
画像
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Functional Studies - Recombinant human AMPK alpha 2 + beta 1 + gamma 2 protein (ab185258)The specific activity of AMPK alpha 2 + beta 1 + gamma 2 (ab185258) was determined to be 297 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human AMPK alpha 2 + beta 1 + gamma 2 protein (ab185258)SDS PAGE analysis of ab185258 -
SDS-PAGE - Recombinant human AMPK alpha 2 + beta 1 + gamma 2 protein (ab185258)SDS PAGE analysis of ab185258 -
SDS-PAGE - Recombinant human AMPK alpha 2 + beta 1 + gamma 2 protein (ab185258)SDS-PAGE analysis of ab185258.
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Functional Studies - Recombinant human AMPK alpha 2 + beta 1 + gamma 2 protein (ab185258)Kinase Assay showing the specific activity of ab185258 as 350 nmol/min/mg.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab185258 は論文での使用が確認できていません。