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AB219442

Recombinant Human Alcohol Dehydrogenase protein (His tag C-Terminus)

Recombinant Human Alcohol Dehydrogenase protein (His tag C-Terminus)

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Recombinant Human Alcohol Dehydrogenase protein (His tag C-Terminus) is a Human Full Length protein, in the 1 to 375 aa range, expressed in Baculovirus infected insect cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

別名を表示する

ADH1, ADH1A, Alcohol dehydrogenase 1A, Alcohol dehydrogenase subunit alpha

1 Images
SDS-PAGE - Recombinant Human Alcohol Dehydrogenase protein (His tag C-Terminus) (AB219442)
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Supplier Data

SDS-PAGE - Recombinant Human Alcohol Dehydrogenase protein (His tag C-Terminus) (AB219442)

15% SDS-PAGE analysis of ab219442 (3μg).

Key facts

精製度

>95% SDS-PAGE

Affinity purified

エンドトキシンレベル

< 1 EU/µg

発現系

Baculovirus infected insect cells

タグ

His tag C-Terminus

アプリケーション

SDS-PAGE

applications

生物活性

No

アクセッション番号

P07327

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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組合せ製品

Proteins & Peptides

AB219448

Recombinant Human ADH1C protein (His tag C-Terminus)

SDS-PAGE - Recombinant Human ADH1C protein (His tag C-Terminus) (AB219448)

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Proteins & Peptides

AB116934

Recombinant Human ADH1B protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ADH1B protein (GST tag N-Terminus) (AB116934)

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Proteins & Peptides

AB124573

Recombinant Human ADH5 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human ADH5 protein (His tag N-Terminus) (AB124573)

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Proteins & Peptides

AB93699

Recombinant human Lactate Dehydrogenase protein (Active) (His tag N-Terminus)

Western blot - Recombinant human Lactate Dehydrogenase protein (Active) (His tag N-Terminus) (AB93699)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"MSTAGKVIKCKAAVLWELKKPFSIEEVEVAPPKAHEVRIKMVAVGICGTDDHVVSGTMVTPLPVILGHEAAGIVESVGEGVTTVKPGDKVIPLAIPQCGKCRICKNPESNYCLKNDVSNPQGTLQDGTSRFTCRRKPIHHFLGISTFSQYTVVDENAVAKIDAASPLEKVCLIGCGFSTGYGSAVNVAKVTPGSTCAVFGLGGVGLSAIMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTMMASLLCCHEACGTSVIVGVPPDSQNLSMNPMLLLTGRTWKGAILGGFKSKECVPKLVADFMAKKFSLDALITHVLPFEKINEGFDLLHSGKSIRTILMFHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"40.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":375,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected insect cells","accessionNumber":"P07327","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Alcohol dehydrogenase (ADH) also known as aldehyde reductase is an enzyme that catalyzes the conversion of alcohols to aldehydes while reducing NAD+ to NADH. This enzyme's structure comprises approximately 40 kDa per subunit and it typically functions as a dimer. ADH is predominantly expressed in the liver where it plays a major role in alcohol metabolism. However its presence is also noted in other tissues such as the stomach lining and kidneys. The enzyme's activities are significant enough to be measured through various assays including ADH assay and alcohol dehydrogenase test highlighting its enzymatic kinetics.
Biological function summary

Alcohol dehydrogenase's role extends beyond simple alcohol metabolism. It participates in the body's detoxification processes. This enzyme is not part of a larger complex but can function alongside other dehydrogenases involved in metabolic processes. ADH's activity helps in managing blood alcohol levels by converting ethanol to acetaldehyde which is further processed by aldehyde dehydrogenase to acetate. The balance and efficiency of these reactions determine the rate and capacity of alcohol clearance from the body.

Pathways

Alcohol dehydrogenase significantly contributes to the alcohol metabolism pathway and the retinol metabolism pathway. It plays a role in converting retinol to retinal which is important for vision and cell growth. In these pathways ADH works closely with aldehyde dehydrogenase to ensure the continuation of the metabolic processes in the human body. The enzyme's function is also connected to the smooth operation of the cytochrome P450 system in the liver for comprehensive detoxification.

The activity of alcohol dehydrogenase links closely to conditions like alcoholism and liver disease. Variations in ADH enzyme activity can affect an individual's susceptibility to alcohol use disorder due to differences in alcohol metabolism rates. Additionally high acetaldehyde levels a product of the alcohol dehydrogenase reaction can contribute to liver damage leading to cirrhosis. This enzyme's relationship to aldehyde dehydrogenase is critical as the failure in converting acetaldehyde can exacerbate the toxic effects and contribute further to these disorders.
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一般的な情報

機能

Alcohol dehydrogenase (PubMed : 2738060). Oxidizes primary as well as secondary alcohols. Ethanol is a very poor substrate (PubMed : 2738060).

配列の類似性

Belongs to the zinc-containing alcohol dehydrogenase family.

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製品プロトコール

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ターゲットの情報

Alcohol dehydrogenase (PubMed : 2738060). Oxidizes primary as well as secondary alcohols. Ethanol is a very poor substrate (PubMed : 2738060).
See full target information ADH1A
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