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Recombinant Human AKT3 protein (ab43621)

Submit a review Q&A (2)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% Densitometry
  • Tags: GST tag N-Terminus
  • Suitable for: WB

製品の詳細

  • 製品名

    Recombinant Human AKT3 protein
    AKT3 タンパク質・ペプチド 製品一覧

  • 精製度

    > 90 % Densitometry.
    Affinity purified.

  • 発現系

    Baculovirus infected Sf9 cells

  • アクセッション番号

    Q9Y243

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 予測される分子量

      84 kDa

    • 領域

      1 to 479

    • タグ

      GST tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab43621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Western blot

  • 製品の状態

    Liquid

  • 備考

    Recombinant full-length human AKT3 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

関連情報

  • 別名

    • AKT
    • Akt3
    • AKT3 kinase
    • AKT3_HUMAN
    • DKFZp434N0250
    • MPPH
    • PKB gamma
    • PKBG
    • PRKBG
    • Protein kinase Akt-3
    • Protein Kinase AKT3
    • Protein kinase B gamma
    • RAC gamma
    • RAC gamma serine/threonine protein kinase
    • RAC-gamma serine/threonine-protein kinase
    • RAC-PK-gamma
    • RACPK Gamma
    • Serine threonine protein kinase Akt 3
    • Serine threonine protein kinase Akt3
    • STK 2
    • STK-2
    • STK2
    • V akt murine thymoma viral oncogene homolog 3
    • V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
    • V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma
    see all

  • 機能

    IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.

  • 組織特異性

    In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.

  • 配列類似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.

  • ドメイン

    Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.

  • 翻訳後修飾

    Phosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.

  • 細胞内局在

    Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab43621 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab43621 は論文での使用が確認できていません。

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Question

I am interested in purchasing AKT1 AB116423 and AKT3 AB43621 for a PPI assay. Please could you confirm: Are these inactive versions of AKT? Are they both His tagged? Many Thanks

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Answer

I have now received additional information from our lab. They have confirmed that the biological activity of ab116423 has not been tested. So I am sorry we will not be able to provide any data regarding this. The ab116423 is double his tagged. ab43621 is GST tagged. In case you are interested in buying active proteins then you may consider the following products; ab79799 / ab60324 ab62279 / ab79792 I hope the information will be helpful choosing the right product. If you need any help with other question or with purchase please do not hesitate to contact me.

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Question

I am interested in purchasing AKT1 AB116423 and AKT3 AB43621 for a PPI assay. Please could you confirm: Are these inactive versions of AKT? Are they both His tagged? Many Thanks

Read More
Answer

Thank you for contacting us. ab43621 is certainly an inactive protein. I have asked our lab to provide more info about ab116423; I will contact you again soon. Many thanks for having patience!

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