AB87670

Recombinant Human ADX protein (T7 tag N-Terminus)

Name: Recombinant Human ADX protein (ab87670) | Abcam
Brand: Abcam Limited
SKU: AB87670
Price: 88000 JPY
Availability: InStock

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(1 Publication)

Recombinant Human ADX protein (T7 tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

別名を表示する

ADX, FDX1, Adrenal ferredoxin, Ferredoxin-1, Hepatoredoxin

1 Images

SDS-PAGE - Recombinant Human ADX protein (T7 tag N-Terminus) (AB87670)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human ADX protein (T7 tag N-Terminus) (AB87670)

15% SDS-PAGE showing ab87670 at approximately 15kDa (3μg).

Key facts

精製度

>90% SDS-PAGE

発現系

Escherichia coli

アプリケーション

SDS-PAGE

applications

生物活性

アクセッション番号

P10109

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

This product was previously labelled as Adrenodoxin

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配列情報

[{"sequence":"MASMTGGQQMGRGSMSSSEDKITVHFINRDGETLTTKGKVGDSLLDVVVENNLDIDGFGACEGTLACSTCHLIFEDHIYEKLDAITDEENDMLDLAYGLTDRSRLGCQICLTKSMDNMTVRVPETVADARQSIDVGKTS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P10109","tags":[{"tag":"T7","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Alternate names for ADX include ADX mouse and FDX1. This target is a protein with a mass of approximately 54 kDa. ADX is expressed in various tissues with a high presence in adrenal cortex and testes. Mechanically it functions as an adrenodoxin which transfers electrons in the adrenal gland mitochondria. As a small iron-sulfur protein ADX interacts with cytochrome P450 enzymes to facilitate steroidogenic reactions.

Biological function summary

ADX plays a significant role in enabling steroid biosynthesis and metabolism acting as an electron donor. ADX is an essential component of the mitochondrial cholesterol side-chain cleavage complex. This complex helps in converting cholesterol into pregnenolone initiating steroid hormone production. It is necessary for producing corticosteroids androgens and estrogens.

Pathways

ADX holds critical functions in the cholesterol and steroid biosynthesis pathways. ADX interacts with the cholesterol transport protein StAR and cytochrome P450scc both essential for initiating the conversion of cholesterol to pregnenolone. Additionally it supports the electron transfer processes necessary for the final conversion steps of various steroid hormones.

Abnormal ADX functioning connects to disorders like congenital adrenal hyperplasia and Addison's disease. These conditions are often linked with insufficient steroid hormone production. Furthermore defects or imbalances in the ADX pathway may involve related proteins such as cytochrome P450 enzymes which could lead to disrupted steroidogenesis and further complications in adrenal gland function.

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製品の性状

製品の状態

Liquid

補足情報

ab87670 is purified using conventional chromatography techniques.Endotoxin: < 1.0 EU per 1 μg of protein (determined by LAL method).

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一般的な情報

機能

Essential for the synthesis of various steroid hormones (PubMed : 20547883, PubMed : 21636783). Participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis (PubMed : 20547883, PubMed : 21636783). Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage (PubMed : 20547883, PubMed : 21636783). Does not form a ternary complex with adrenodoxin reductase and CYP11A1 but shuttles between the two enzymes to transfer electrons (By similarity).

配列の類似性

Belongs to the adrenodoxin/putidaredoxin family.

細胞内局在性

Mitochondrion matrix

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information FDX1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

British journal of pharmacology 182:2409-2425 PubMed39933533

2025

Targeting FDX1 by trilobatin to inhibit cuproptosis in doxorubicin-induced cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Wei,Guozhen Lan,Wenfang Zhang,Wang Ran,Yu Wei,Xin Liu,Yuandong Zhang,Qihai Gong,Haibo Li,Jianmei Gao

View all publications

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Abcam product promise

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Recombinant Human ADX protein (T7 tag N-Terminus)