Recombinant Human Activin Receptor Type IA protein (Fc Chimera) (ab83922)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant Human Activin Receptor Type IA protein (Fc Chimera)
Activin Receptor Type IA タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE. -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
Theoretical Sequence MEDEKPKVNPKLYMCVCEGLSCGNEDHCEGQQCFSSLSINDGFHVYQKGC FQVYEQGKMTCKTPPSPGQAVECCQGDWCNRNITAQLPTKGKSFPGTQNF HLEGSSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTL MISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYR VVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTL PPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSD GSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK -
領域
1 to 123 -
タグ
Fc tag C-Terminus -
配列の追加情報
Fused with the Fc region of Human IgG1 at the C-terminus.
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特性
Our Abpromise guarantee covers the use of ab83922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituents: 10% Trehalose, 1% Human serum albumin
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再構成It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
関連情報
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別名
- Activin A receptor type I
- Activin A receptor type II like kinase 2
- Activin receptor type I
see all -
機能
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin. May be involved for left-right pattern formation during embryogenesis. -
組織特異性
Expressed in normal parenchymal cells, endothelial cells, fibroblasts and tumor-derived epithelial cells. -
関連疾患
Defects in ACVR1 are a cause of fibrodysplasia ossificans progressiva (FOP) [MIM:135100]. FOP is a rare autosomal dominant disorder of skeletal malformations and progressive extraskeletal ossification. Heterotopic ossification in FOP begins in childhood and can be induced by trauma or may occur without warning. Bone formation is episodic and progressive, leading to extra-articular ankylosis of all major joints of the axial and appendicular skeleton, rendering movement impossible. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 GS domain.
Contains 1 protein kinase domain. -
細胞内局在
Membrane. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human Activin Receptor Type IA protein (Fc Chimera) (ab83922)Lane 1 – MW markers; Lane 2 – ab83922; Lane 3 – ab83922 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83922 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 μg of protein was loaded per lane; Gel was stained using Coomassie.
Drop in MW after treatment with PNGase F indicates the presence of N-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes. -
SDS-PAGE - Recombinant Human Activin Receptor Type IA protein (Fc Chimera) (ab83922)A sample of ab83922 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Approximately 40 μg of protein was loaded; Gel was stained using Deep Purple™. The spot train indicates the presence of multiple glycoforms of ab83922. Spots within the spot train were cut from the gel and identified as Activin Receptor Type IA (Fc Chimera) by protein mass fingerprinting. -
Functional Studies - Recombinant Human Activin Receptor Type IA protein (Fc Chimera) (ab83922)Post-translational modifications result in protein heterogeneity. The densitometry scan demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification.
The triangle indicates theoretical pI and MW of the protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab83922 は論文での使用が確認できていません。
