Recombinant Human ACE2 protein (ab151852)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, HPLC
製品の詳細
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製品名
Recombinant Human ACE2 protein
ACE2 タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGD KWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLN TILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWES WRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDY SRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLL GDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVS VGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMD DFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKH LKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGE IPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYT RTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWT LALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQS IKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGE EDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRL NDNSLEFLGIQPTLGPPNQPPVSVDHHHHHH -
予測される分子量
85 kDa including tags -
領域
18 to 740 -
タグ
His tag C-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab151852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
HPLC
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.001% Zinc chloride, 0.32% Tris HCl, 10% Glycerol, 0.88% Sodium chloride
関連情報
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別名
- ACE 2
- ACE related carboxypeptidase
- ACE-related carboxypeptidase
see all -
機能
Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. -
組織特異性
Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. -
配列類似性
Belongs to the peptidase M2 family. -
翻訳後修飾
N-glycosylation on Asn-90 may limit SARS infectivity. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
画像
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Western blot - Recombinant Human ACE2 protein (ab151852)Recombinant Human ACE2 protein (ab151852) + Human ACE-2 Protein (C-His), reducing condition
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab151852 は 15 報の論文で使用されています。
- Lebedin M et al. Discriminating promiscuous from target-specific autoantibodies in COVID-19. Eur J Immunol 53:e2250210 (2023). PubMed: 36856018
- Maher S et al. Gold conjugated nanobodies in a signal-enhanced lateral flow test strip for rapid detection of SARS-CoV-2 S1 antigen in saliva samples. Sci Rep 13:10643 (2023). PubMed: 37391465
- Laotee S et al. CHO-produced RBD-Fc subunit vaccines with alternative adjuvants generate immune responses against SARS-CoV-2. PLoS One 18:e0288486 (2023). PubMed: 37450510
- Casciola-Rosen L et al. IgM anti-ACE2 autoantibodies in severe COVID-19 activate complement and perturb vascular endothelial function. JCI Insight 7:N/A (2022). PubMed: 35349483
- Khajeh Pour S et al. Plasma angiotensin peptides as biomarkers of rheumatoid arthritis are correlated with anti-ACE2 auto-antibodies level and disease intensity. Inflammopharmacology 30:1295-1302 (2022). PubMed: 35618976