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AB123190

Recombinant Human ABHD14B protein (His tag N-Terminus)

Recombinant Human ABHD14B protein (His tag N-Terminus)

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Recombinant Human ABHD14B protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 210 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

別名を表示する

CIB, ABHD14B, Putative protein-lysine deacylase ABHD14B, Alpha/beta hydrolase domain-containing protein 14B, CCG1-interacting factor B, Abhydrolase domain-containing protein 14B

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SDS-PAGE - Recombinant Human ABHD14B protein (His tag N-Terminus) (AB123190)
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Supplier Data

SDS-PAGE - Recombinant Human ABHD14B protein (His tag N-Terminus) (AB123190)

SDS-PAGE analysis of Recombinant Human ABHD14B protein (ab123190), under reducing conditions. Proteins visualized by coomassie blue stain.

Lane 1 : Molecular Weight Standards

Lane 2 : 3 μg Recombinant Human ABHD14B protein

Key facts

精製度

>95% SDS-PAGE

ab123190 is purified using conventional chromatography techniques.

発現系

Escherichia coli

タグ

His tag N-Terminus

アプリケーション

SDS-PAGE, Mass Spec

applications

生物活性

No

アクセッション番号

Q96IU4

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

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組合せ製品

Primary Antibodies

AB276068

Anti-ABHD14B antibody [011] - BSA and Azide free

Recombinant

Immunoprecipitation - Anti-ABHD14B antibody [011] - BSA and Azide free (AB276068)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMAASVEQREGTIQVQGQALFFREALPGSGQARFSVLLLHGIRFSSETWQNLGTLHRLAQAGYRAVAIDLPGLGHSKEAAAPAPIGELAPGSFLAAVVDALELGPPVVISPSLSGMYSLPFLTAPGSQLPGFVPVAPICTDKINAANYASVKTPALIVYGDQDPMGQTSFEHLKQLPNHRVLIMKGAGHPCYLDKPEEWHTGLLDFLQGLQ","proteinLength":"Full Length","predictedMolecularWeight":"25 kDa","actualMolecularWeight":null,"aminoAcidEnd":210,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96IU4","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
出荷温度
Blue Ice
短期保存温度
-20°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein ABHD14B also known as abhydrolase domain containing 14B has a significant role in biochemical processes. It is about 42 kDa in mass. The ABHD14B protein functions as a serine hydrolase which means it belongs to a broad category of enzymes that hydrolyze ester bonds. It is expressed mostly in the liver and brain indicating its roles in various cellular functions in these tissues.
Biological function summary

ABHD14B plays a part in cellular metabolism and signal transduction. It interacts with other proteins within a multi-protein complex to regulate lipid homeostasis influencing the production and breakdown of lipid molecules. ABHD14B enzymes modulate these processes which are essential for maintaining cell membrane integrity and signaling events that control cell growth and differentiation.

Pathways

The involvement of ABHD14B includes the lipid signaling and metabolic regulation pathways. It has connections with the fatty acid metabolism pathway coordinating with proteins like ACC and FASN to manage fatty acid synthesis and degradation. Its activities impact lipid synthesis and degradation showing its importance in maintaining cellular energy balance and signaling.

Improper function of ABHD14B links to metabolic diseases such as hepatic steatosis also known as fatty liver disease. It also holds connections to neurological disorders owing to its expression in the brain. Proteins involved in these conditions like ACC in metabolic disorders or Tau in neurological diseases interact with ABHD14B indicating its potential role in these pathologies. Understanding these interactions may provide insights into therapeutic targets.
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一般的な情報

機能

Acts as an atypical protein-lysine deacetylase in vitro (PubMed : 31478652). Catalyzes the deacetylation of lysine residues using CoA as substrate, generating acetyl-CoA and the free amine of protein-lysine residues (PubMed : 31478652). Additional experiments are however required to confirm the protein-lysine deacetylase activity in vivo (Probable). Has hydrolase activity towards various surrogate p-nitrophenyl (pNp) substrates, such as pNp-butyrate, pNp-acetate and pNp-octanoate in vitro, with a strong preference for pNp-acetate (PubMed : 14672934, PubMed : 31478652). May activate transcription (PubMed : 14672934).

配列の類似性

Belongs to the AB hydrolase superfamily. ABHD14 family.

細胞内局在性

Nucleus

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製品プロトコール

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ターゲットの情報

Acts as an atypical protein-lysine deacetylase in vitro (PubMed : 31478652). Catalyzes the deacetylation of lysine residues using CoA as substrate, generating acetyl-CoA and the free amine of protein-lysine residues (PubMed : 31478652). Additional experiments are however required to confirm the protein-lysine deacetylase activity in vivo (Probable). Has hydrolase activity towards various surrogate p-nitrophenyl (pNp) substrates, such as pNp-butyrate, pNp-acetate and pNp-octanoate in vitro, with a strong preference for pNp-acetate (PubMed : 14672934, PubMed : 31478652). May activate transcription (PubMed : 14672934).
See full target information ABHD14B
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