Recombinant Human ABCB5 protein (Tagged) (ab185403)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Suitable for: HPLC, SDS-PAGE
製品の詳細
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製品名
Recombinant Human ABCB5 protein (Tagged) -
精製度
> 95 % SDS-PAGE.
ab185403 is greater than 95% pure as determined by SEC-HPLC and reducing SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEY QGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQL KEFLDANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPD LGTDDDDKAMAIRSADLIVTLKDGMLAEKGAHAELMAKRGLYYSLVMSQD IKKADEQMESMTYSTERKTNSLPLHSVKSIKSDFIDKAEESTQSKEISLP EVSLLKILKL NKPEWPFV -
予測される分子量
29 kDa including tags -
領域
141 to 247 -
配列の追加情報
Trx tag at the N-terminus.
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特性
Our Abpromise guarantee covers the use of ab185403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
HPLC
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 100% PBS -
再構成Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 3X PBS. Please aliquot the reconstituted solution. After reconstitution aliquot and store at < -20 degrees, avoid freeze/thaw cycles.
関連情報
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別名
- ABCB 5
- ABCB5
- ABCB5 P-gp
see all -
機能
Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. -
組織特異性
Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Also expressed in several malignant tissues. -
配列類似性
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 2 ABC transporter domains. -
細胞内局在
Cell membrane. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab185403 は論文での使用が確認できていません。
