Recombinant human 2B4 protein (Fc Chimera Active) (ab185251)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, ELISA
製品の詳細
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製品名
Recombinant human 2B4 protein (Fc Chimera Active)
2B4 タンパク質・ペプチド 製品一覧 -
生理活性
Measured by its binding ability in a functional ELISA. Immobilized Hab185251 at 2 μg/mL (100 μL/well) can bind Human CD48, Mouse IgG2a Fc Tag with a linear range of 2-16 ng/mL.
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精製度
> 95 % SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
CQGSADHVVSISGVPLQLQPNSIQTKVDSIAWKKLLPSQNGFHHILKWEN GSLPSNTSNDRFSFIVKNLSLLIKAAQQQDSGLYCLEVTSISGKVQTATF QVFVFDKVEKPRLQGQGKILDRGRCQVALSCLVSRDGNVSYAWYRGSKLI QTAGNLTYLDEEVDINGTHTYTCNVSNPVSWESHTLNLTQDCQNAHQEFR -
予測される分子量
49 kDa including tags -
領域
22 to 221 -
タグ
Fc tag C-Terminus -
配列の追加情報
Fused with Fc fragment of Human IgG1 at the C-terminus (NP_057466).
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特性
Our Abpromise guarantee covers the use of ab185251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
ELISA
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon reconsitution add a carrier protein (0.1% BSA). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.61% Tris, 0.75% Glycine, 0.877% Sodium chloride, 0.436% L-ArginineThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
関連情報
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別名
- 2B4
- C9.1
- CD244
see all -
機能
Modulate other receptor-ligand interactions to enhance leukocyte activation. -
組織特異性
Expressed in spleen, PBL, followed by lung, liver, testis and small intestine. Expressed not only in NK cells, but also on monocytes and basophils. -
配列類似性
Contains 2 Ig-like (immunoglobulin-like) domains. -
細胞内局在
Membrane. - Information by UniProt
画像
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SDS-PAGE - Recombinant human 2B4 protein (Fc Chimera Active) (ab185251)
Human 2B4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The protein migrates as 60-80 kDa due to glycosylation.
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ELISA - Recombinant human 2B4 protein (Fc Chimera Active) (ab185251)Immobilized Hab185251 at 2 μg/mL (100 μL/well) can bind Human CD48, Mouse IgG2a Fc Tag with a linear range of 2-16 ng/mL.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab185251 は論文での使用が確認できていません。
