Recombinant Human 14-3-3 zeta protein (ab87361)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant Human 14-3-3 zeta protein
14-3-3 zeta タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE.
Purified by using conventional chromatography techniques. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMDK NELVQKAKLA EQAERYDDMA ACMKSVTEQG AELSNEERNL LSVAYKNVVG ARRSSWRVVS SIEQKTEGAE KKQQMAREYR EKIETELRDI CNDVLSLLEK FLIPNASQAE SKVFYLKMKG DYYRYLAEVA AGDDKKGIVD QSQQAYQEAF EISKKEMQPT HPIRLGLALN FSVFYYEILN SPEKACSLAK TAFDEAIAEL DTLSEESYKD STLIMQLLRD NLTLWTSDTQ GDEAEAGEGG EN -
予測される分子量
32 kDa -
領域
1 to 245 -
タグ
His tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab87361 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: PBS
関連情報
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別名
- 14 3 3 delta
- 14 3 3 protein zeta/delta
- 14 3 3 protein/cytosolic phospholipase A2
see all -
機能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
配列類似性
Belongs to the 14-3-3 family. -
翻訳後修飾
The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. This phosphorylation appears to be activated by sphingosine. Phosphorylation on Thr-232; inhibits binding of RAF1. -
細胞内局在
Cytoplasm. Melanosome. Located to stage I to stage IV melanosomes. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human 14-3-3 zeta protein (ab87361)ab87361 on 15% SDS-PAGE (3µg)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab87361 は 3 報の論文で使用されています。
- Kong D et al. Procoxacin bidirectionally inhibits osteoblastic and osteoclastic activity in bone and suppresses bone metastasis of prostate cancer. J Exp Clin Cancer Res 42:45 (2023). PubMed: 36759880
- Ruff SE et al. PIM1 phosphorylation of the androgen receptor and 14-3-3 ? regulates gene transcription in prostate cancer. Commun Biol 4:1221 (2021). PubMed: 34697370
- Hou Y et al. Effect of cytokine-induced killer cells combined with dendritic cells on the survival rate and expression of 14-3-3? and p-Bad proteins in Lewis lung cancer cell lines. Oncol Lett 16:1815-1820 (2018). PubMed: 30008870
