Recombinant Human 14-3-3 Theta + Tau protein (ab116444)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% Purified via His tag
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
製品の詳細
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製品名
Recombinant Human 14-3-3 Theta + Tau protein
14-3-3 Theta + Tau タンパク質・ペプチド 製品一覧 -
精製度
> 80 % Purified via His tag.
ab116444 was purified via Ni2+-NTA agarose columns. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMEKTELIQKAKLAEQA ERYDDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIE QKTDTSDKKLQLIKDYREKVESELRSICTTVLELLDKYLIANATNPESKV FYLKMKGDYFRYLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPI RLGLALNFSVFYYEILNNPELACTLAKTAFDEAIAELDTLNEDSYKDSTL IMQLLRDNLTLWTSDSAGEECDAAEGAEN -
予測される分子量
31 kDa including tags -
領域
1 to 245 -
タグ
His tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab116444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Mass Spectrometry
SDS-PAGE
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質量分析
MALDI-TOF-TOF -
製品の状態
Liquid -
備考
ab116444 was alkylated, digested with trypsin and the mass of the resultant peptides determined by MADLI-TOF/TOF mass spectrometry. The peptides obtained gave 64 % sequence coverage of 14-3-3 tau + theta. Theoretical pI: 5.2
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.012% Benzamidine, 0.004% PMSF, 0.02% DTT, 0.6% HEPES, 50% Glycerol
関連情報
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別名
- 14-3-3 protein T cell
- 14-3-3 protein T-cell
- 14-3-3 protein tau
see all -
機能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
組織特異性
Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement. -
配列類似性
Belongs to the 14-3-3 family. -
翻訳後修飾
Ser-232 is probably phosphorylated by CK1. -
細胞内局在
Cytoplasm. In neurons, axonally transported to the nerve terminals. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human 14-3-3 Theta + Tau protein (ab116444)12% SDS-PAGE gel showing ab116444.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab116444 は論文での使用が確認できていません。