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Recombinant Human 14-3-3 gamma/YWHAG protein (ab53869)

Submit a review Q&A (1)
SDS-PAGE - Recombinant Human 14-3-3 gamma/YWHAG protein (ab53869)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Suitable for: SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant Human 14-3-3 gamma/YWHAG protein
      14-3-3 gamma/YWHAG タンパク質・ペプチド 製品一覧

    • 精製度

      > 95 % SDS-PAGE.
      Recombinant human 14-3-3 was expressed in E.coli and purified by using conventional chromatography techniques.

    • エンドトキシン・レベル

      < 1.000 Eu/µg

    • 発現系

      Escherichia coli

    • アクセッション番号

      P61981

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MVDREQLVQK ARLAEQAERY DDMAAAMKNV TELNEPLSNE ERNLLSVAYK NVVGARRSSW RVISSIEQKT SADGNEKKIE MVRAYREKIE KELEAVCQDV LSLLDNYLIK NCSETQYESK VFYLKMKGDY YRYLAEVATG EKRATVVESS EKAYSEAHEI SKEHMQPTHP IRLGLALNYS VFYYEIQNAP EQACHLAKTA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDD DGGEGNN

      • 予測される分子量

        28 kDa

      • 領域

        1 to 247

    特性

    Our Abpromise guarantee covers the use of ab53869 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      SDS-PAGE

    • 製品の状態

      Liquid

    • 備考

      This product was previously labelled as 14-3-3 gamma.

    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 7.40
      Constituents: PBS, 10% Glycerol (glycerin, glycerine)

    関連情報

    • 別名

      • 14 3 3 gamma
      • 14 3 3 protein gamma
      • 14 3 3 protein gamma subtype
      • 14 3 3gamma
      • 14-3-3 protein gamma
      • 1433G_HUMAN
      • 3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide
      • KCIP 1
      • KCIP-1
      • KCIP1
      • N-terminally processed
      • Protein kinase C inhibitor protein 1
      • Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein gamma polypeptide
      • Ywhag
      see all

    • 機能

      Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

    • 組織特異性

      Highly expressed in brain, skeletal muscle, and heart.

    • 配列類似性

      Belongs to the 14-3-3 family.

    • 翻訳後修飾

      Phosphorylated by various PKC isozymes.

    • 細胞内局在

      Cytoplasm.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human 14-3-3 gamma/YWHAG protein (ab53869)
      SDS-PAGE - Recombinant Human 14-3-3 gamma/YWHAG protein (ab53869)

      3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab53869 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab53869 は論文での使用が確認できていません。

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    What kind of chromatography is used to purify the protein? If the protein is tagged, what kind of tag is it and where is the tag?

    Read More
    Answer

    Thank you for your call yesterday. I have heard back from the lab, and the protein ab53869 is not tagged. The protein is purified via a proprietary chromatography technique that does not require a tag. I hope this information is helpful, but please let me know if there is anything else that we can do for you.

    Read More

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