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AB53869

Recombinant Human 14-3-3 gamma/YWHAG protein (Tag Free)

Recombinant Human 14-3-3 gamma/YWHAG protein (Tag Free)

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Recombinant Human 14-3-3 gamma/YWHAG protein (Tag Free) is a Human Full Length protein, in the 1 to 247 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

別名を表示する

14-3-3 protein gamma, Protein kinase C inhibitor protein 1, KCIP-1, YWHAG

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SDS-PAGE - Recombinant Human 14-3-3 gamma/YWHAG protein (Tag Free) (AB53869)
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Supplier Data

SDS-PAGE - Recombinant Human 14-3-3 gamma/YWHAG protein (Tag Free) (AB53869)

3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.

Key facts

精製度

>95% SDS-PAGE

Recombinant human 14-3-3 was expressed in E.coli and purified by using conventional chromatography techniques.

エンドトキシンレベル

< 1 EU/µg

発現系

Escherichia coli

タグ

Tag free

アプリケーション

SDS-PAGE

applications

生物活性

No

アクセッション番号

P61981

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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組合せ製品

Reagents, Controls & Accessories

AB116029

Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (5 - 245 kDa) (AB116029)

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Proteins & Peptides

AB87361

Recombinant Human 14-3-3 zeta protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human 14-3-3 zeta protein (His tag N-Terminus) (AB87361)

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  • 0
Proteins & Peptides

AB276453

Recombinant Human SMOC1 protein (His tag)

SDS-PAGE - Recombinant Human SMOC1 protein (His tag) (AB276453)

  • 0
  • 0
Proteins & Peptides

AB80033

Recombinant human Hsp90 beta protein (Active) (Tag Free)

Western blot - Recombinant human Hsp90 beta protein (Active) (Tag Free) (AB80033)

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Proteins & Peptides

AB85273

Recombinant Human 14-3-3 alpha + beta protein (Tag Free)

SDS-PAGE - Recombinant Human 14-3-3 alpha + beta protein (AB85273)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

This product was previously labelled as 14-3-3 gamma.
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配列情報

[{"linker":null,"sequence":"MVDREQLVQKARLAEQAERYDDMAAAMKNVTELNEPLSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTSADGNEKKIEMVRAYREKIEKELEAVCQDVLSLLDNYLIKNCSETQYESKVFYLKMKGDYYRYLAEVATGEKRATVVESSEKAYSEAHEISKEHMQPTHPIRLGLALNYSVFYYEIQNAPEQACHLAKTAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDDDGGEGNN","proteinLength":"Full Length","predictedMolecularWeight":"28 kDa","actualMolecularWeight":null,"aminoAcidEnd":247,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P61981","tags":[]}]
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出荷温度及び保存条件

製品の状態
Liquid
出荷温度
Blue Ice
短期保存温度
-20°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

14-3-3 gamma also known as YWHAG or 14-3-3 gamma protein is a member of the 14-3-3 protein family. These proteins play significant roles in signal transduction. 14-3-3 gamma is a dimeric protein with a molecular mass of about 28 to 32 kDa per monomer. It is ubiquitously expressed including in tissues such as brain muscle and heart. This protein influences a wide range of cellular processes by interacting with various signaling proteins often through recognition of phosphorylated serine or threonine motifs.
Biological function summary

Proteins within the 14-3-3 family like the 14-3-3 gamma function as adaptors and scaffolds in signal transduction pathways. They are part of multi-protein complexes that modulate interactions among proteins within the cell. 14-3-3 gamma interacts dynamically with a variety of target proteins assisting in their activities and proper localization. Its interaction facilitates cellular processes including cell cycle control apoptosis and stress response owing to its activity as a regulator of distinct signaling pathways.

Pathways

14-3-3 gamma participates in the MAPK/ERK pathway which is pivotal for cell growth and differentiation. It also plays an integral role in the PI3K/AKT pathway which contributes to survival and proliferation signals. In these pathways 14-3-3 gamma interacts with important kinases such as Raf and AKT. These interactions help transduce signals from receptors on the cell surface to the nucleus impacting gene expression and cellular responses.

Research has linked 14-3-3 gamma to neurodegenerative diseases such as Alzheimer's disease. In Alzheimer's 14-3-3 gamma interacts with proteins like tau which become abnormally phosphorylated and aggregate. Additionally this protein has associations with certain cancers where it might influence tumor growth and progression through interactions with cell cycle regulatory proteins. Its modulation of key signaling pathways makes it a candidate of interest in the development of therapeutic strategies for these diseases.
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一般的な情報

機能

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 15696159, PubMed : 16511572, PubMed : 36732624). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 15696159, PubMed : 16511572, PubMed : 36732624). Binding generally results in the modulation of the activity of the binding partner (PubMed : 16511572). Promotes inactivation of WDR24 component of the GATOR2 complex by binding to phosphorylated WDR24 (PubMed : 36732624). Participates in the positive regulation of NMDA glutamate receptor activity by promoting the L-glutamate secretion through interaction with BEST1 (PubMed : 29121962). Reduces keratinocyte intercellular adhesion, via interacting with PKP1 and sequestering it in the cytoplasm, thereby reducing its incorporation into desmosomes (PubMed : 29678907). Plays a role in mitochondrial protein catabolic process (also named MALM) that promotes the degradation of damaged proteins inside mitochondria (PubMed : 22532927).

配列の類似性

Belongs to the 14-3-3 family.

翻訳後修飾

Phosphorylated by various PKC isozymes.

細胞内局在性

Mitochondrion matrix

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製品プロトコール

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ターゲットの情報

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed : 15696159, PubMed : 16511572, PubMed : 36732624). Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (PubMed : 15696159, PubMed : 16511572, PubMed : 36732624). Binding generally results in the modulation of the activity of the binding partner (PubMed : 16511572). Promotes inactivation of WDR24 component of the GATOR2 complex by binding to phosphorylated WDR24 (PubMed : 36732624). Participates in the positive regulation of NMDA glutamate receptor activity by promoting the L-glutamate secretion through interaction with BEST1 (PubMed : 29121962). Reduces keratinocyte intercellular adhesion, via interacting with PKP1 and sequestering it in the cytoplasm, thereby reducing its incorporation into desmosomes (PubMed : 29678907). Plays a role in mitochondrial protein catabolic process (also named MALM) that promotes the degradation of damaged proteins inside mitochondria (PubMed : 22532927).
See full target information YWHAG
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