Recombinant Human 14-3-3 eta/YWHAH protein (ab116442)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% Purified via His tag
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
製品の詳細
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製品名
Recombinant Human 14-3-3 eta/YWHAH protein
14-3-3 eta/YWHAH タンパク質・ペプチド 製品一覧 -
精製度
> 80 % Purified via His tag.
ab116442 was purified by Ni2+-NTA agarose -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMGDREQLLQRARLAEQ AERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVISSI EQKTMADGNEKKLEKVKAYREKIEKELETVCNDVLSLLDKFLIKNCNDFQ YESKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISKEQMQ PTHPIRLGLALNFSVFYYEIQNAPEQACLLAKQAFDDAIAELDTLNEDSY KDSTLIMQLLRDNLTLWTSDQQDEEAGEGN -
予測される分子量
32 kDa including tags -
領域
1 to 246 -
タグ
His tag N-Terminus
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特性
Our Abpromise guarantee covers the use of ab116442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Mass Spectrometry
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質量分析
MALDI-TOF-TOF -
製品の状態
Liquid -
備考
ab116442 was alkylated, digested with trypsin and the mass of the resultant peptides determined by MADLI-TOF/TOF. The peptides obtained gave 67% sequence coverage of 14-3-3 eta/YWHAH. Theoretical pI: 5.3
This product was previously labelled as 14-3-3 eta
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.012% Benzamidine, 0.003% PMSF, 0.02% DTT, 0.6% HEPES, 50% Glycerol
関連情報
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別名
- 14 3 3 protein eta
- 14-3-3 eta
- 14-3-3 protein eta
see all -
機能
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. -
組織特異性
Expressed mainly in the brain and present in other tissues albeit at lower levels. -
配列類似性
Belongs to the 14-3-3 family. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human 14-3-3 eta/YWHAH protein (ab116442)12 % SDS-PAGE gel showing ab116442.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab116442 は論文での使用が確認できていません。