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Recombinant HSV1 gD protein (ab48969)

Submit a review Q&A (1)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Suitable for: ELISA, WB

製品の詳細

  • 製品名

    Recombinant HSV1 gD protein
    HSV1 gD タンパク質・ペプチド 製品一覧

  • 精製度

    > 95 % SDS-PAGE.

  • 発現系

    Escherichia coli

  • タンパク質長

    Protein fragment

  • Animal free

    No

  • 由来

    Recombinant

    • 配列の追加情報

      E.coli derived recombinant protein. The protein contains the HSV-1 gD immunodominant regions (226-394aa).

特性

Our Abpromise guarantee covers the use of ab48969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    ELISA

    Western blot

  • 製品の状態

    Liquid
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% Urea, 0.395% Tris HCl, 0.0292% EDTA, 50% Glycerol (glycerin, glycerine)

関連情報

  • 別名

    • GD
    • Glycoprotein D
    • Herpes simplex virus type 1 glycoprotein D
    • HSV1 glycoprotein D

  • 関連性

    Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell. Glycoprotein D (gD) has been implicated in binding to cellular receptors that facilitate virus penetration into cells. Herpes simplex virus type 1 (HSV-1) glycoprotein D (gD) is an essential component of the entry apparatus that is responsible for viral penetration and subsequent cell-cell spread.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab48969 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab48969 は論文での使用が確認できていません。

レビューと Q&A

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Question

Customer kindly inquiring about specific protocol used to validate ab43045, ab48969, ab48970 in WB and ELISA.

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Answer

Thank you for contacting Abcam.

For ab43045, we do not have the specific protocol information that validated this protein in WB and ELISA. We update the product datasheets with tested applications and species based on a variety of sources, whether it is our own lab work, specific references, or abreviews posted by researchers demonstrating successful use of the product in a particular application or species.

For ab48969 and ab48970, 2 micrograms of the protein were loaded in a SDS PAGE gel and a standard western blot protocol was followed. We do not have the specific protocol used to validate the protein in ELISA.

I'm sorry that this protocol information isn't more specific, but we do guarantee all 3 of these proteins to work in both western blot and ELISA as stated on the datasheet and will provide scientific support if there are any issues. If we cannot work with you to solve the issues, we will happily replace the product or refund your purchase within 6 months of purchase. Further details about our AbPromise guarantee are given through the link below:
https://www.abcam.com/index.html?pageconfig=resource&rid=10372&pid=10000&source=pagetrap

Our recommended western blot and ELISA protocols are given through the links below, and we also recommend optimizing the use of these proteins for both western blot and ELISA for each researcher's specific experimental conditions.
Western blotting: https://www.abcam.com/index.html?pageconfig=resource&rid=11375
ELISA (direct, indirect, fluorescent substrate, primary antibody): https://www.abcam.com/index.html?pageconfig=popular_protocols

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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