Recombinant HIV1 Nef protein (ab185584)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His-T7 tag N-Terminus
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant HIV1 Nef protein
HIV1 Nef タンパク質・ペプチド 製品一覧 -
精製度
> 90 % SDS-PAGE.
The final product was refolded using temperature shift inclusion body refolding technology and chromatographically purified. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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配列
MASMTGGQQMGRGHHHHHHENLYFQGGFGGKWSKSSVIGWPAVRERMRRA EPAADGVGAVSRDLEKHGAITSSNTAANNAACAWLEAQEEEEVGFPVTPQ VPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPD WQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTSLLHPVSLHGM DDPEREVLEWRFDSRLAFHHVARELHPEYFKNC -
予測される分子量
26 kDa including tags -
領域
1 to 206 -
タグ
His-T7 tag N-Terminus -
配列の追加情報
Derived from NL4-3 strain and constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal.
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関連製品
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Related Products
- Anti-TEV Tag antibody [KT88] (ab179875)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-HIV1 Nef antibody [3F2] (ab25866)
- Anti-HIV1 Nef antibody [3D12] (ab42355)
- Anti-HIV1 Nef antibody [JR6] (ab42358)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
- Anti-T7 tag® antibody (ab9115)
特性
Our Abpromise guarantee covers the use of ab185584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituent: 0.32% Tris HCl
Contains NaCl, KCl, EDTA, arginine, DTT and Sucrose.
関連情報
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別名
- C terminal core protein
- F protein
- Nef
see all -
関連性
Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab185584 は 1 報の論文で使用されています。
- Yan Q et al. Inhibition of Kaposi's sarcoma-associated herpesvirus lytic replication by HIV-1 Nef and cellular microRNA hsa-miR-1258. J Virol 88:4987-5000 (2014). PubMed: 24554664
