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Recombinant Hepatitis C Virus NS3 protein (ab84346)

Submit a review Q&A (8)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% Affinity purified
  • Tags: His tag N-Terminus
  • Suitable for: Functional Studies

製品の詳細

  • 製品名

    Recombinant Hepatitis C Virus NS3 protein
    Hepatitis C Virus NS3 タンパク質・ペプチド 製品一覧

  • 生理活性

    1 unit equals 1 nanogram of purified protein.

  • 精製度

    > 95 % Affinity purified.
    Recombinant ab84346 was expressed in a E.coli system and purified by an affinity column in combination with FPLC chromatography. The purified protein is greater than 95% homogeneous and contains no detectable DNase and RNase activity.

  • 発現系

    Escherichia coli

  • タンパク質長

    Protein fragment

  • Animal free

    No

  • 由来

    Recombinant

    • 予測される分子量

      23 kDa

    • タグ

      His tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab84346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

  • 製品の状態

    Liquid

  • 備考

    1 unit equals 1 nanogram of purified protein.
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.9
    Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)

関連情報

  • 別名

    • Hepatitis C Virus NS3
    • NS3
    • NS3P
    • p70
    • Serine protease/NTPase/helicase
    see all

  • 関連性

    HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.

  • 細胞内局在

    Endoplasmic reticulum membrane

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab84346 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab84346 は論文での使用が確認できていません。

レビューと Q&A

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レビューを送る 質問を送る

1-8 of 8 Abreviews or Q&A

Question

Please, what is the most suitable HCV NS3 recombinant protein to use as positive control in WB applications with ab40464?

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Answer

Thank you for contacting us.

Please accept my apologies for the delay, it was a very comprehensive search.

Unfortunately, I have to let you know that we do not have a suitable HCV NS3 protein available that can be used as a positive control in Western blot with ab40464.

This antibody was generated against genotype 1b-HCV NS3 protein aa1027-1219, the epitope was mapped to aa1186-1219. None of our recombinant HCV NS3 proteins contain this region though.

I am sorry that I cannot be of more assistance on this occasion, but I hope this information is still helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

I have one question want to confirm with you.

We ordered ab 84346 twice from my lab. The first time we got 100ul, this time 10ug, 0.5ug/ul, 20ul.

Could you check the record and verify for me? Because I need to know for my experiment.

Read More
Answer

Thank you for your inquiry.

According to our records you ordered ab84346 on order 1130723 in July 2012 and received lot GR20464-3, 10ug at 0.5 mg/ml, so 20 ul total. Then ab84346 was reordered on order 1182382 and you received the same lot GR20464-5 (the last number is different since it was aliquoted later, but it is the same lot), so you still received 10ug at 0.5 mg/ml, so 20 ul total.

We have never sold a 100ul version of ab84346 according to our records.

I hope this information helps. Please contact us with any other questions.

Read More

Question

We are interested in your proteins, and we need your advises for choosing one of them. We need full-length NS3 (helicase)from HCV, which have adouble strand DNA unwinding activity for our experiments. Is there a NS3 protein with its unwinding activity?

Read More
Answer

Following my message from yesterday, I was informed by our source of the NS3/NS4a protein, ab84346, that helicase activity has not been tested.

I am sorry we could not be of more help. If you have any questions, please contact us. Good luck in your research.

Read More

Question

We are interested in your proteins, and we need your advises for choosing one of them. We need full-length NS3 (helicase) from HCV, which have a double strand DNA unwindingactivity for our experiments (please refer attached file). Is there a NS3 protein with its unwinding activity? Actually, I interested in ab84346 but I am not sure it has helicase activity, and work for me. I think there is also a same question (in scientific support tap in linked page, july 2012) about the helicase activity. Could you please check and let me know it has a activity or not? and is there another protein without NS4A fragment? actually we need only active full-length NS3.

Read More
Answer

Thank you for contacting us.

The product ab84346 has not been tested for helicase activity but I have asked our source for the protein to confirm. I will forward what I receive from them.

We do not have a full-length NS3. There is another product, ab123463, which has proteolytic activity but not helicase activity. It is also a fragment, not full-length NS3.
.

Read More

Question


I read it. Even this is not exactly what described about ab84346. But if it is really as you said in this email, it should work for me.

I care about the NS3. It seems NS3 is full length. I want to test helicase. I do not wish NS3 is short truncated form.

I believe this product includes NS3 helicase domain, right?

Read More
Answer

Thank you for your reply.

Yes, this protein will have both the Helicase ATP-binding (a.a. 1223 - 1375) and Helicase C-terminal (a.a. 1382 - 1544) domains.

http://www.uniprot.org/uniprot/Q81258

I apologize for the confusion, and we have since updated the datasheet. Thank you for bringing this to our attention.

I hope this information helps. Please contact us with any other questions.

Read More

Question

Is this the Hepatitis C Virus NS3 protein Recombinant full length Hepatitis C Virus (strain NZL1) NS3 protein, or HCV NS3/4A protease?

Read More
Answer

Thank you for your inquiry.

I heard back from the lab that this product is a fusion protein combined NS4A and NS3 fragments with it protease activity. There is a 6x His tag on the N-terminal side of NS4A.

http://www.uniprot.org/uniprot/Q81258

So according to SwissProt, the sequence would be from a.a. 1033-1717 and include the Serine protease NS3 + Non-structural protein 4A.

Is this okay to use in your study? I know that the description wasn't very clear on the website, so please let me know if this information affects you and I will be able to replace or refund your order. I look forward to your reply.

Read More

Question

Would like to test this antibody in a helicase activity assay.

Read More
Answer

DISCOUNT CODE: ***
Expiration date: November 19, 2012

This code will give you 1 free protein before the expiration date. To redeem this offer, please submit an Abreview forab84346 in a helicase functionalstudyand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question

Dear Abcam,

I was wondering if your recombinant HCV NS3 protein (ab84346 or ab91395) contains an additional N-terminal methionine or if it is the wild-type sequence starting with an alanine? Since it is recombinant I assume you have added M in order to start translation.

Regards,

Read More
Answer

Thank you for your enquiry and for your patience.

I can confirm that both these recombinant HCV NS3 proteins (ab84346 and ab91395) contain an additionalN terminal Methionine.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

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