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Recombinant Hepatitis C Virus genotype 1a NS5 protein (ab49033)

Submit a review Q&A (1)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Tags: GST tag N-Terminus
  • Suitable for: ELISA, WB, Flow Cyt

製品の詳細

  • 製品名

    Recombinant Hepatitis C Virus genotype 1a NS5 protein

  • 精製度

    > 95 % SDS-PAGE.

  • 発現系

    Escherichia coli

  • タンパク質長

    Protein fragment

  • Animal free

    No

  • 由来

    Recombinant

    • 領域

      2212 to 2313

    • タグ

      GST tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab49033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    ELISA

    Western blot

    Flow Cytometry

  • 製品の状態

    Liquid

  • 備考

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.

    pH: 7.2
    Constituents: 0.2% Triton-X-100, 9% Urea, 0.395% Tris HCl, 50% Glycerol (glycerin, glycerine)

関連情報

  • 別名

    • HCV 1a NS5
    • Hepatitis C virus NS5A
    • Non-structural protein 5A
    • p56

  • 関連性

    Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha. NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It's exact role is not currently known (2008). NS5B (non-structural protein 5B) is an RNA-dependant RNA polymerase responsible for replication of the hepatitis C viral genome, and is currently a principal target for chemotherapeutic inhibition of HCV replication. Hepatitis C virus (HCV) can cause chronic hepatitis, cirrhosis and hepatocellular carcinoma. At present there is no vaccine effective against HCV.

  • 細胞内局在

    Endoplasmic reticulum membrane

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab49033 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab49033 は論文での使用が確認できていません。

レビューと Q&A

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Question


I’ve attached the protocol I used for the Western blot. I’m trying to find a good antibody to HCV NS5A so that I can select stable clones overexpressing the protein. So far, I haven’t been able to pick up any NS5A using two proteins from Abcam, ab49033 and ab68096, and high concentrations (1:100, 1:250) of four different antibodies (ab20342, sc-57776, ab20773, ab2594).

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Answer

Thanks for sending along your protocol.

1. ab49033 is unlikely to be detected by these antibodies as it only contains about 100 aa of the total protein. As described on datasheet this product containsamino acids"corresponding to amino acids 2212-2313 of Hepatitis C Virus NS5" The epitopes recognized by these antibodies would have to recognize NS5A within this small region to work.

2. Usingponceau S stain post transfer, have you been able to determine protein is present on membrane after transfer?

3. Regarding your protocol, a) Boiling of samplesis not recommended; instead heat at 70 deg C for 10min prior to loading gel b) Try blocking with 5% BSA instead of milk c) Use 1:1000 for primary antibodies and 1:5000 for secondary antibodies.

I look forward to hearing if these suggestions prove useful.

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