Recombinant HDAC3+NCOR2 complex protein (ab42631)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: Inhibition Assay
製品の詳細
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製品名
Recombinant HDAC3+NCOR2 complex protein -
生理活性
Activity: 110 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 uM Biomol substrate (Catalog number KI177), and 1 ng/ul HDAC3/NcoR2. Incubation condition: 30 min at 37ºC.
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精製度
> 90 % SDS-PAGE.
Affinity purified. -
発現系
Baculovirus infected insect cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant
関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab42631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Inhibition Assay
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製品の状態
Liquid -
備考
Co-expressed in a Baculovirus infected Sf9 cell expression system. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.614% Glutathione, 0.79% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.8004% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- CTG26
- HD 3
- HD3
see all -
機能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the histone deacetylase family. HD type 1 subfamily. -
翻訳後修飾
Sumoylated in vitro. -
細胞内局在
Nucleus. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab42631 は 2 報の論文で使用されています。
- Fan Z et al. Identification of SIRT3 as an eraser of H4K16la. iScience 26:107757 (2023). PubMed: 37720100
- Demmerle J et al. The nuclear envelope protein emerin binds directly to histone deacetylase 3 (HDAC3) and activates HDAC3 activity. J Biol Chem : (2012). PubMed: 22570481