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AB63819

Recombinant E. coli RuvA protein (Active)

Recombinant E. coli RuvA protein (Active)

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Recombinant E. coli RuvA protein (Active) is a Escherichia coli K-12 Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.

別名を表示する

b1861, JW1850, ruvA, Holliday junction branch migration complex subunit RuvA, Ruv A, Holliday junction DNA helicase RuvA, Holliday junction ATP dependent DNA helicase ruvA

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SDS-PAGE - Recombinant E. coli RuvA protein (Active) (AB63819)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant E. coli RuvA protein (Active) (AB63819)

SDS-PAGE analysis of Recombinant E. coli RuvA protein (ab63819).

Key facts

精製度

>90% SDS-PAGE

purified by methods such as chromatography

発現系

Escherichia coli

タグ

Tag free

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Active

アクセッション番号

P0A809

アニマルフリー

No

キャリアフリー

No

種

Escherichia coli K-12

バッファー組成

pH: 6 - 8.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.158% Tris HCl, 0.0584% EDTA, 0.039% 2-Mercaptoethanol

storage-buffer

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組合せ製品

Reagents, Controls & Accessories

AB325997

Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3-250 kDa, 5-color) (AB325997)

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Proteins & Peptides

AB63827

Recombinant E. coli RuvB protein (Active)

SDS-PAGE - Recombinant E. coli RuvB protein (Active) (AB63827)

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Proteins & Peptides

AB63828

Recombinant E. coli RuvC protein (Active)

SDS-PAGE - Recombinant E. coli RuvC protein (Active) (AB63828)

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Proteins & Peptides

AB63816

Recombinant E. coli LexA protein

SDS-PAGE - Recombinant E. coli LexA protein (AB63816)

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Proteins & Peptides

AB174091

Recombinant E. coli RecA protein (Active)

SDS-PAGE - Recombinant E. coli RecA protein (Active) (AB174091)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Functional as Holliday junction specific binding protein, which promotes Holliday-junction branch migration in combination with RuvB protein.</p>" } } }
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製品の詳細

This protein may be suitable for the following applications. 1) Studies on homologous recombination mechanism. 2) For SNP analysis. 3) Incorporation to DNA circuit. 4) Recognition and identification of cross-like DNA.
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配列情報

[{"linker":null,"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P0A809","tags":[]}]
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出荷温度及び保存条件

製品の状態
Liquid
出荷温度
Blue Ice
短期保存温度
-20°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RuvA also known as Holliday junction ATP-binding protein or Holliday junction helicase RuvA is a protein with a molecular mass of approximately 22 kDa. It plays a critical role in DNA repair and recombination processes. RuvA is expressed primarily in prokaryotic organisms where it functions to recognize and bind the Holliday junction a cruciform structure formed during homologous recombination and DNA repair. RuvA facilitates the branch migration of these junctions by stabilizing them allowing other proteins to process them during DNA strand exchange and repair mechanisms.
Biological function summary

The functioning of RuvA enables the stabilization and resolution of the Holliday junctions. RuvA operates as part of a complex with the proteins RuvB and RuvC together forming the RuvABC complex. RuvB acts as a motor protein by utilizing ATP to drive the branch migration process while RuvC resolves the junction by cleaving DNA strands. RuvA binding to the junction catalyzes the formation of this complex ensuring efficient resolution of recombination intermediates essential for accurate DNA repair and genetic recombination.

Pathways

RuvA is integral to the RecBCD and RecFOR pathways which are central DNA repair and homologous recombination pathways. RuvA assists in the late stages of recombination and interacts closely with RuvB and RuvC to promote the migration and resolution of Holliday junctions. These pathways ensure the maintenance of genomic stability and participate in processes for repairing double-strand breaks and facilitating proper chromosomal segregation during cell division.

RuvA dysfunction or perturbation can lead to genomic instability increasing the risk of conditions like cancer. Although RuvA is a prokaryotic protein and not directly associated with human diseases its function informs our understanding of similar pathways in eukaryotic cells like those involving the XPG protein which is part of the DNA repair pathway. Insights into RuvA and its interactions with proteins such as RuvB and RuvC contribute to a broader understanding of repair mechanisms that when defective are implicated in various genomic disorders and the development of certain cancers.
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一般的な情報

機能

The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair (PubMed : 21531731, PubMed : 6374379). The RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR); RFR and homologous recombination required for UV light survival can be separated (PubMed : 16424908, PubMed : 18942176, PubMed : 21531731, PubMed : 9814711). The RuvA-RuvB complex promotes Holliday junction (HJ) branch migration. RuvA binds to HJ cruciform DNA, conferring on it an open structure (PubMed : 10890893, PubMed : 7885479, PubMed : 9628481). In the presence of RuvB, ATP and Mg(2+) the junction is dissociated; hydrolyzable (d)NTPs can replace ATP but other analogs cannot (PubMed : 10772859, PubMed : 10890893, PubMed : 1608954, PubMed : 1617728, PubMed : 1833759, PubMed : 7885479, PubMed : 8393934, PubMed : 9628481). The RuvB hexamer acts as a pump, pulling dsDNA into and through the RuvAB complex (PubMed : 9078376). Can bypass UV-induced lesions (PubMed : 1617728) and physically cross-linked DNA strands (PubMed : 10662672). RuvA gives specificity by binding to cruciform junctions, while the RuvB ATPase provides the motor force for branch migration; excess RuvB can promote branch migration in the absence of RuvA (PubMed : 10662672, PubMed : 1617728). Overexpression of RuvA alone leads to UV sensitivity (PubMed : 2164626). RuvA stimulates the weak ATPase activity of RuvB in the presence of DNA; the addition of HJ DNA further stimulates ATPase about 10-fold (PubMed : 1435721, PubMed : 1833759, PubMed : 8393934). Inhibits RuvC endoDNase activity by binding to HJ DNA, including DNA to which RuvC is already bound (PubMed : 9000618). Does not bind dsDNA (PubMed : 11080172). May act as a collar that slides at HJ which promotes branch migration while inhibiting other DNA remodeling activities (Probable).. An in vitro resolvase system that forms and processes HJ has been reconstituted with DNA substrates, RuvA, RuvB and RuvC. RuvA-RuvB increases the rate of strand exchange (branch migration), dissociates the RecA filament and allows RuvC to cleave in both orientations at the cruciform junction (PubMed : 10421637, PubMed : 9160752). HJ-RuvA-RuvB-RuvC complexes resolve Holliday junctions and also undergo branch migration, providing evidence for a coupled branch migration/HJ resolution reaction (PubMed : 10421637).

配列の類似性

Belongs to the RuvA family.

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製品プロトコール

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ターゲットの情報

The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair (PubMed : 21531731, PubMed : 6374379). The RuvA-RuvB complex plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR); RFR and homologous recombination required for UV light survival can be separated (PubMed : 16424908, PubMed : 18942176, PubMed : 21531731, PubMed : 9814711). The RuvA-RuvB complex promotes Holliday junction (HJ) branch migration. RuvA binds to HJ cruciform DNA, conferring on it an open structure (PubMed : 10890893, PubMed : 7885479, PubMed : 9628481). In the presence of RuvB, ATP and Mg(2+) the junction is dissociated; hydrolyzable (d)NTPs can replace ATP but other analogs cannot (PubMed : 10772859, PubMed : 10890893, PubMed : 1608954, PubMed : 1617728, PubMed : 1833759, PubMed : 7885479, PubMed : 8393934, PubMed : 9628481). The RuvB hexamer acts as a pump, pulling dsDNA into and through the RuvAB complex (PubMed : 9078376). Can bypass UV-induced lesions (PubMed : 1617728) and physically cross-linked DNA strands (PubMed : 10662672). RuvA gives specificity by binding to cruciform junctions, while the RuvB ATPase provides the motor force for branch migration; excess RuvB can promote branch migration in the absence of RuvA (PubMed : 10662672, PubMed : 1617728). Overexpression of RuvA alone leads to UV sensitivity (PubMed : 2164626). RuvA stimulates the weak ATPase activity of RuvB in the presence of DNA; the addition of HJ DNA further stimulates ATPase about 10-fold (PubMed : 1435721, PubMed : 1833759, PubMed : 8393934). Inhibits RuvC endoDNase activity by binding to HJ DNA, including DNA to which RuvC is already bound (PubMed : 9000618). Does not bind dsDNA (PubMed : 11080172). May act as a collar that slides at HJ which promotes branch migration while inhibiting other DNA remodeling activities (Probable).. An in vitro resolvase system that forms and processes HJ has been reconstituted with DNA substrates, RuvA, RuvB and RuvC. RuvA-RuvB increases the rate of strand exchange (branch migration), dissociates the RecA filament and allows RuvC to cleave in both orientations at the cruciform junction (PubMed : 10421637, PubMed : 9160752). HJ-RuvA-RuvB-RuvC complexes resolve Holliday junctions and also undergo branch migration, providing evidence for a coupled branch migration/HJ resolution reaction (PubMed : 10421637).
See full target information ruvA
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