Recombinant E. coli RecA protein (Active) (ab174091)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: Functional Studies, SDS-PAGE, Electron Microscopy
製品の詳細
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製品名
Recombinant E. coli RecA protein (Active)
RecA タンパク質・ペプチド 製品一覧 -
精製度
> 90 % SDS-PAGE.
ab174091 was over-expressed as a recombinant protein and highly purified by several steps of chromatography. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Escherichia coli -
配列
MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTGSLSLDI ALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHAL DPIYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAAL TPKAEIEGEIGDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKI GVMFGNPETTTGGNALKFYASVRLDIRRIGAVKEGENVVGSETRVKVVKN KIAAPFKQAEFQILYGEGINFYGELVDLGVKEKLIEKAGAWYSYKGEKIG QGKANATAWLKDNPETAKEIEKKVRELLLSNPNSTPDFSVDDSEGVAETN EDF -
予測される分子量
36 kDa -
領域
1 to 353
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製品の詳細
Recombinant E. coli RecA protein (Active)
関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab174091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
Electron Microscopy
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 0.32% Tris-HCl buffer, 0.03% EDTA, 1.1% Potassium chloride, 0.02% DTT
関連情報
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別名
- DNA strand exchange and recombination protein with protease and nuclease activity
- lexB
- Protein recA
see all -
関連性
RecA in E.coli can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage. -
細胞内局在
Cytoplasmic
画像
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SDS-PAGE - Recombinant E. coli RecA protein (Active) (ab174091)
SDS-PAGE analysis of purified Recombinant E. coli RecA protein (ab174091).
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SDS-PAGE - Recombinant E. coli RecA protein (Active) (ab174091)Polyacrylamide gel electrophoresis of ab174091.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab174091 は 1 報の論文で使用されています。
- Horii T et al. Regulation of SOS functions: purification of E. coli LexA protein and determination of its specific site cleaved by the RecA protein. Cell 27:515-22 (1981). Functional Studies ; Escherichia coli . PubMed: 6101204