Recombinant E. coli groEL protein (ab113176)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: WB, EIA, Functional Studies, ELISA
製品の詳細
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製品名
Recombinant E. coli groEL protein
groEL タンパク質・ペプチド 製品一覧 -
精製度
> 90 % SDS-PAGE.
ab113176 is purified by multi-step chromatography. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Escherichia coli -
配列
AAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITK DGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIIT EGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTIS ANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLS PYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIA EDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVI SEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQ QIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALH ATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPL RQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTR SALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM -
予測される分子量
57 kDa -
領域
2 to 548 -
配列の追加情報
Strain K12.
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製品の詳細
Recombinant E. coli groEL protein
関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab113176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Enzyme Immunoassay
Functional Studies
ELISA
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: 0.58% Sodium chloride, 0.79% Tris HCl, 0.08% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.1% Magnesium chloride
関連情報
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別名
- 60 kDa chaperonin
- groEL protein
- groL
see all -
関連性
The bacterial chaperonin groEL is a double toroidal assembly, which together with the action of the ring-shaped oligomeric cochaperonin, GroES, facilitates protein folding in an ATP dependent manner. -
細胞内局在
Cytoplasmic
画像
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SDS-PAGE analysis of ab113176.
Lane 1: MW marker
Lane 2: 0.5 μg of ab113176 (groEL protein)
Lane 3: 1 μg of ab113176 (groEL protein)
Lane 4: 2 μg of ab113176 (groEL protein)
Lane 5: 5 μg of ab113176 (groEL protein)
Detected by Coomassie stain. -
Western blot analysis using ab113176.
Lane 1: MW marker
Lane 2: 100 ng of Recombinant E. coli groEL protein (ab113176) probed with an anti-groEL monoclonal antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab113176 は論文での使用が確認できていません。