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Recombinant E. coli fur protein (ab78816)

Submit a review Q&A (6)
SDS-PAGE - Recombinant <em>E. coli </em> fur protein (ab78816)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Suitable for: SDS-PAGE

    製品の詳細

    • 製品名

      Recombinant E. coli fur protein

    • 精製度

      > 95 % SDS-PAGE.
      ab78816 is purified using conventional chromatography techniques.

    • 発現系

      Escherichia coli

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Escherichia coli

      • 配列

        MTDNNTALKK AGLKVTLPRL KILEVLQEPD NHHVSAEDLY KRLIDMGEEI GLATVYRVLN QFDDAGIVTR HNFEGGKSVF ELTQQHHHDH LICLDCGKVI EFSDDSIEAR QREIAAKHGI RLTNHSLYLY GHCAEGDCRE DEHAHEGK

    • 製品の詳細

      Recombinant E. coli fur protein

    特性

    Our Abpromise guarantee covers the use of ab78816 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      SDS-PAGE

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.0222% Calcium chloride, 0.00174% PMSF, 0.242% Tris, 0.58% Sodium chloride

    関連情報

    • 別名

      • DNA binding transcriptional dual regulator of siderophore biosynthesis and transport
      • ECK0671
      • Ferric uptake regulation protein
      • Ferric uptake regulator
      • FUR
      • JW0669
      see all

    • 関連性

      fur (ferric uptake regulator) protein is a DNA-binding protein which regulates iron-responsive genes. A small, 17-kDa, global transcriptional repressor, fur, in the presence of iron regulates functions as diverse as iron acquisition, oxidative stress, and virulence. In Escherichia coli, members of the fur family regulate the expression of more than 100 genes that function in processes as varied as the biosynthesis and transport of siderophores, the expression of virulence factors, the alleviation of oxidative and NO-induced stress, and the inhibition of ferritin production through the expression of RyhB.

    • 細胞内局在

      Cytoplasmic

    画像

    • SDS-PAGE - Recombinant <em>E. coli </em> fur protein (ab78816)
      SDS-PAGE - Recombinant E. coli fur protein (ab78816)
      15% SDS-PAGE showing ab78816 at approximately 16kDa (3µg).

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab78816 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab78816 は論文での使用が確認できていません。

    レビューと Q&A

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    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Question

    PMSF is commonly used during protein extraction of the Fur protein to prevent degradation during purification. However, it is not normally added to the buffer post-purification.The protein should not degrade if conserved ina sterilepure buffer after purification.

    To rest conform to what is described in the literature, itis preferable to avoid adding PMSF to the purified protein if possible.

    Read More
    Answer

    Thank you for your email.

    I will pass on your comments to the lab and hopefully your request can be accommodated.

    I will be in touch.

    Read More

    Question

    Yes, there was one other vial, ordered earlier in Oct/Nov with the same degradation pattern, thus, a total of 3 to be replaced.

    Read More
    Answer

    Thank you for your email.

    I will arrange for the replacement of the 3 vials as soon as a new lot is available.

    I will be in touch and I apologize for all the trouble and inconvenience.

    Read More

    Question

    As I orderedseveral tubes, would it be possible to havea replacement of the total quantity ordered? If so, then yes, I would like a replacement.

    Read More
    Answer

    Thank you for your reply.

    Yes, I'd be happy to replace all those vials that did not work.

    I have found one order from xxxx.

    Please let me know if there were any other orders where the vials were showing degradation. If not, I will arrange the free of charge replacement for the 2 vials mentioned above.

    I look forward to hear back from you and resolve this case.

    Read More

    Question

    I recently ordered your Fur protein, reference ab78816, which was aliquoted at placed at -20°C upon reception, as recommended. The concentration is fine, however when run on a 12% SDS-Page gel, multiple bands are visible near 17kDa, the expected protein size, and the estimated purity is below 95% (˜75% if including all three bands, 40% if including only the largest).
    In the associated documents sent by Abcam, only one band can be seen on a 15% gel. I have attached a copy of my SDS-page gel for reference. Have you previously obtained a similar pattern, or do these additional bands represent degradation products?
    Can you also confirm that the protein has not been previously exposed to denaturing agents?

    Read More
    Answer

    Thank you for your patience.

    The lab retested the 2lots and the protein is indeed degraded. We are very sorry for this inconvenience and apologize about any problems caused.

    A new lot would be available in about 2-3 weeks.

    I can offer you either a free of charge replacement with the new lot, a credit or refund. Please let me know which option would be best for you.

    I look forward to hear back and resolve this problem for you.

    Read More

    Question

    I recently ordered your Fur protein, reference ab78816, which was aliquoted at placed at -20°C upon reception, as recommended. The concentration is fine, however when run on a 12% SDS-Page gel, multiple bands are visible near 17kDa, the expected protein size, and the estimated purity is below 95% (˜75% if including all three bands, 40% if including only the largest).
    In the associated documents sent by Abcam, only one band can be seen on a 15% gel. I have attached a copy of my SDS-page gel for reference. Have you previously obtained a similar pattern, or do these additional bands represent degradation products?
    Can you also confirm that the protein has not been previously exposed to denaturing agents?

    Read More
    Answer

    Thank you for contacting us.

    I have heard back from the lab with the following information:

    We haven not obtained a similar pattern. We will verify whether these additional bands represent degradation by re-testing them today.

    The protein has not been exposed to denaturing agents.
    As soon as the lab has the result of the SDS-PAGE, I will email you.
    In the meanwhile, please contact me without hesitation if you have any additional questions.

    Read More

    Question

    I recently ordered your Fur protein, reference ab78816, which was aliquoted at placed at -20°C upon reception, as recommended. The concentration is fine, however when run on a 12% SDS-Page gel, multiple bands are visible near 17kDa, the expected protein size, and the estimated purity is below 95% (˜75% if including all three bands, 40% if including only the largest).
    In the associated documents sent by Abcam, only one band can be seen on a 15% gel. I have attached a copy of my SDS-page gel for reference. Have you previously obtained a similar pattern, or do these additional bands represent degradation products?
    Can you also confirm that the protein has not been previously exposed to denaturing agents?

    Read More
    Answer

    Thank you for contacting us.

    I am sorry to hear about the problems encountered with this protein.

    I will contact the lab with your data and inquire about QC data for these lots.

    Do you see the same band pattern for both vials? Please let me know.

    I will be in touch with more information from the lab very soon. I also look forward to hear back from you.

    Read More

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