AB51121

Recombinant DnaK protein (Tag Free)

Name: Recombinant DnaK protein (ab51121) | Abcam
Brand: Abcam Limited
SKU: AB51121
Price: 56000 JPY
Availability: InStock

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(2 Publications)

Recombinant DnaK protein (Tag Free) is a Escherichia coli K-12 Full Length protein, in the 1 to 638 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

別名を表示する

groP, grpF, seg, b0014, JW0013, dnaK, Chaperone protein DnaK, HSP70, Heat shock 70 kDa protein, Heat shock protein 70

1 Images

SDS-PAGE - Recombinant DnaK protein (Tag Free) (AB51121)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant DnaK protein (Tag Free) (AB51121)

15% SDS-PAGE

Key facts

精製度

>85% SDS-PAGE

発現系

Escherichia coli

アプリケーション

SDS-PAGE

applications

生物活性

アクセッション番号

P0A6Y8

アニマルフリー

キャリアフリー

種

Escherichia coli K-12

バッファー組成

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"sequence":"MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAWVEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDIDDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLLDVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAADNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLNEDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIESALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDDDVVDAEFEEVKDKK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":638,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P0A6Y8","tags":[]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Plays an essential role in the initiation of phage lambda DNA replication, where it acts in an ATP-dependent fashion with the DnaJ protein to release lambda O and P proteins from the preprimosomal complex. DnaK is also involved in chromosomal DNA replication, possibly through an analogous interaction with the DnaA protein. Also participates actively in the response to hyperosmotic shock.

配列の類似性

Belongs to the heat shock protein 70 family.

翻訳後修飾

Autophosphorylated; GrpE inhibits the autophosphorylation.. Can be UMPylated on a tyrosine residue by SelO/YdiU, probably under stress conditions.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information dnaK

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33858942

2021

Killed whole-genome reduced-bacteria surface-expressed coronavirus fusion peptide vaccines protect against disease in a porcine model.

Applications

Unspecified application

Species

Unspecified reactive species

Denicar Lina Nascimento Fabris Maeda,Debin Tian,Hanna Yu,Nakul Dar,Vignesh Rajasekaran,Sarah Meng,Hassan M Mahsoub,Harini Sooryanarain,Bo Wang,C Lynn Heffron,Anna Hassebroek,Tanya LeRoith,Xiang-Jin Meng,Steven L Zeichner

Molecular cell 70:614-627.e7 PubMed29754824

2018

CnoX Is a Chaperedoxin: A Holdase that Protects Its Substrates from Irreversible Oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Camille V Goemans,Didier Vertommen,Rym Agrebi,Jean-François Collet

View all publications

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Abcam product promise

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Recombinant DnaK protein (Tag Free)