AB322310

Recombinant Cynolmogus Monkey Apolipoprotein A-I/APOA1 Protein (His-tag)

Name: Recombinant Cynolmogus Monkey Apolipoprotein A-I/APOA1 Protein (His-tag) (ab322310) | Abcam
Brand: Abcam Limited
SKU: AB322310
Price: 17000 JPY
Availability: InStock

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Recombinant Cynolmogus Monkey Apolipoprotein A-I/APOA1 Protein (His-tag) is a Cynomolgus monkey Full Length protein, expressed in Escherichia coli, with >95%, <0.005 EU/µg endotoxin level, suitable for Mass Spec, SDS-PAGE.

別名を表示する

Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1

1 Images

SDS-PAGE - Recombinant Cynolmogus Monkey Apolipoprotein A-I/APOA1 Protein (His-tag) (AB322310)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Cynolmogus Monkey Apolipoprotein A-I/APOA1 Protein (His-tag) (AB322310)

SDS-PAGE analysis of ab322310 under reducing conditions for 2ug protein.

Key facts

精製度

>95% HPLC

エンドトキシンレベル

<0.005 EU/µg

発現系

Escherichia coli

アプリケーション

Mass Spec, SDS-PAGE

applications

生物活性

質量分析

LC-MS/MS

アクセッション番号

P68292

アニマルフリー

Yes

キャリアフリー

種

Cynomolgus monkey

再構成

Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.

バッファー組成

pH: 7.4 Constituents: PBS, 5% Trehalose

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"linker":null,"sequence":"DEPPQTPWDRVKDLVTVYVEALKDSGKDYVSQFEGSALGKQLNLKLLDNWDSVTSTVSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELHEGTRQKLHELHEKLSPLGEEVRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKASEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLSTQ","proteinLength":"Full Length","predictedMolecularWeight":"29.69 kDa","actualMolecularWeight":"29.56 kDa","aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P68292","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

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出荷温度及び保存条件

製品の状態

Lyophilized

出荷温度

Blue Ice

短期保存温度

Ambient

長期保存温度

Ambient

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Apolipoprotein A-I (ApoA-I) is a major component of high-density lipoprotein (HDL) particles often referred to as 'good cholesterol'. It has a molecular mass of approximately 28 kDa. ApoA-I is mainly expressed in the liver and intestine. It plays a mechanical role in the reverse transport of cholesterol acting to facilitate the efflux of cholesterol from tissues to the liver for excretion. Its alternative names include ApoAI ApoA1 and a component of the AI kits used for measuring this protein.

Biological function summary

ApoA-I functions in cholesterol homeostasis and inflammation. It is a structural component of the HDL complex that mobilizes cholesterol. ApoA-I acts as an activator of the enzyme lecithin-cholesterol acyltransferase (LCAT) which is essential for the maturation of HDL particles. This maturation is necessary for effective cholesterol transport and clearance. ApoA-I's ability to stabilize HDL particles and enhance their functionality makes it significant for maintaining lipid balance and cellular homeostasis.

Pathways

The interaction of ApoA-I with HDL formation and function marks its role in lipid metabolism pathways. Its participation in the reverse cholesterol transport pathway highlights its influence on cardiovascular health. ApoA-I also interacts with other proteins like ApoA-II and paraoxonase-1 which further influence lipid metabolism and antioxidant activities. Understanding these relationships helps elucidate the dynamics of cholesterol removal from the bloodstream.

Disturbances in ApoA-I levels correlate with cardiovascular disease and atherosclerosis. Deficiency or dysfunction in ApoA-I can impair HDL function leading to poor cholesterol removal and buildup within arteries. It is also connected to amyloidosis where misfolded ApoA-I forms deposits in tissues. Understanding these pathological conditions helps researchers target ApoA-I in therapeutic strategies to mitigate disease progression often studying it alongside proteins like ApoB which is associated with low-density lipoprotein (LDL) particles.

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一般的な情報

機能

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

配列の類似性

Belongs to the apolipoprotein A1/A4/E family.

翻訳後修飾

Glycosylated.. Palmitoylated.. Phosphorylation sites are present in the extracellular medium.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information APOA1

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Abcam product promise

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Recombinant Cynolmogus Monkey Apolipoprotein A-I/APOA1 Protein (His-tag)