Native Staphylococcus aureus Protein A (HRP) (ab7456)
Key features and details
- Expression system: Native
- Purity: > 95% n/a
- Suitable for: WB, Dot blot, ELISA, IHC-P, Immunomicroscopy
製品の詳細
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製品名
Native Staphylococcus aureus Protein A (HRP)
Protein A タンパク質・ペプチド 製品一覧 -
精製度
> 95 % n/a.
Protein A Peroxidase Conjugation was prepared from chromatographically pure Protein A. -
発現系
Native -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Native -
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生物種
Staphylococcus aureus -
予測される分子量
56 kDa
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標識
HRP
特性
Our Abpromise guarantee covers the use of ab7456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Dot blot
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunomicroscopy
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製品の状態
Liquid -
備考
Molecular Weight (protein A): 42,000 daltons
Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14)
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C.
pH: 6.5
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride, 1% BSA
関連情報
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別名
- gG-binding protein A
- Immunoglobulin G binding protein A
- SPA
- Staphylococcal protein A
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関連性
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. -
細胞内局在
Secreted; cell wall.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (19)
ab7456 は 19 報の論文で使用されています。
- Chen S et al. Assembly of Immunogenic Protein Particles toward Advanced Synthetic Vaccines. Small 19:e2205819 (2023). PubMed: 36564365
- Viard J et al. Chr21 protein-protein interactions: enrichment in proteins involved in intellectual disability, autism, and late-onset Alzheimer's disease. Life Sci Alliance 5:N/A (2022). PubMed: 35914814
- Won M et al. ANKRD13a controls early cell-death checkpoint by interacting with RIP1 independent of NF-κB. Cell Death Differ 29:1152-1163 (2022). PubMed: 34839354
- Kuninaga N et al. Serological and histological evaluation of species-specific immunocontraceptive vaccine antigens based on zona pellucida 3 in the small Indian mongoose (Herpestes auropunctatus). J Vet Med Sci 81:328-337 (2019). PubMed: 30587673
- Mahajan D et al. Dopey1-Mon2 complex binds to dual-lipids and recruits kinesin-1 for membrane trafficking. Nat Commun 10:3218 (2019). PubMed: 31324769