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AB7345

Native Staphylococcus aureus Protein A (Alkaline Phosphatase)

Native Staphylococcus aureus Protein A (Alkaline Phosphatase)

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Native Staphylococcus aureus Protein A (Alkaline Phosphatase) is a Staphylococcus aureus subsp. aureus NCTC 8325 Full Length protein, expressed in Native, with >95%, suitable for ELISA, WB, Dot, IHC-Fr, IHC-P.

別名を表示する

SAOUHSC_00069, spa, Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA

Key facts

精製度

>95%

発現系

Native

タグ

Tag free

アプリケーション

WB, ELISA, IHC-P, Dot, IHC-Fr

applications

生物活性

No

標識

Alkaline Phosphatase

励起波長/蛍光波長
アクセッション番号

P02976

アニマルフリー

No

キャリアフリー

No

種

Staphylococcus aureus subsp. aureus NCTC 8325

バッファー組成

Preservative: 0.1% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1.5% BSA, 0.87% Sodium chloride, 0.79% Tris HCl, 0.0095% Magnesium chloride, 0.00136% Zinc chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Protein A Concentration: 1.0 mg/ml (by UV absorbance at 280 nm) This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the stock concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.
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配列情報

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"56 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Native","expressionSystem":null,"accessionNumber":"P02976","tags":[]}]
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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
False
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製品の性状

製品の状態

Liquid

補足情報

Protein A Alkaline Phosphatase Conjugated was prepared from chromatographically pure Protein A.

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一般的な情報

機能

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).

配列の類似性

Belongs to the immunoglobulin-binding protein SpA family.

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製品プロトコール

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ターゲットの情報

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
See full target information spa
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組合せ製品

Proteins & Peptides

AB52213

Recombinant Protein A + Protein G

SDS-PAGE - Recombinant Protein A + Protein G (AB52213)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB18596

HRP Anti-Protein A antibody

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88

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