Native Staphylococcus aureus Protein A (ab7399)
Key features and details
- Expression system: Native
- Purity: > 95% n/a
- Suitable for: ELISA, WB
製品の詳細
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製品名
Native Staphylococcus aureus Protein A
Protein A タンパク質・ペプチド 製品一覧 -
精製度
> 95 % n/a.
Protein A is chromatographically pure and shows predominantly a single band by SDS-PAGE. -
発現系
Native -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Native -
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生物種
Staphylococcus aureus -
予測される分子量
56 kDa
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特性
Our Abpromise guarantee covers the use of ab7399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
ELISA
Western blot
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製品の状態
Liquid -
備考
Molecular Weight: 42,000 daltons
Extinction Coefficient: 0.1% at 280 nm is 0.14 (E0.1% of 0.14)
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
関連情報
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別名
- gG-binding protein A
- Immunoglobulin G binding protein A
- SPA
- Staphylococcal protein A
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関連性
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. -
細胞内局在
Secreted; cell wall.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab7399 は 4 報の論文で使用されています。
- Zhang P et al. Evanescent scattering imaging of single protein binding kinetics and DNA conformation changes. Nat Commun 13:2298 (2022). PubMed: 35484120
- Romih T et al. The Effect of Preconditioning Strategies on the Adsorption of Model Proteins onto Screen-Printed Carbon Electrodes. Sensors (Basel) 22:N/A (2022). PubMed: 35684806
- Hsu MN et al. Smart Hydrogel Microfluidics for Single-Cell Multiplexed Secretomic Analysis with High Sensitivity. Small 14:e1802918 (2018). PubMed: 30334375
- Hong LTA et al. An injectable hydrogel enhances tissue repair after spinal cord injury by promoting extracellular matrix remodeling. Nat Commun 8:533 (2017). PubMed: 28912446